Syntrophic acetate oxidation during the two-phase anaerobic digestion of waste activated sludge: Microbial population, Gibbs free energy and kinetic modelling

2017 ◽  
Vol 125 ◽  
pp. 177-188 ◽  
Author(s):  
Daniele Montecchio ◽  
Giovanni Esposito ◽  
Maria Cristina Gagliano ◽  
Agata Gallipoli ◽  
Andrea Gianico ◽  
...  
Keyword(s):  
Free Energy ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Gibbs Free Energy ◽  
Microbial Population ◽  
Kinetic Modelling ◽  
Waste Activated Sludge ◽  
Acetate Oxidation ◽  
Two Phase ◽  
Syntrophic Acetate Oxidation

scholarly journals Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge

2013 ◽  
Vol 79 (20) ◽  
pp. 6491-6500 ◽  
Author(s):  
Dang P. Ho ◽  
Paul D. Jensen ◽  
Damien J. Batstone
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Retention Time ◽  
Elevated Temperatures ◽  
Stable Carbon Isotope ◽  
High Rate ◽  
Continuous Reactor ◽  
Waste Activated Sludge ◽  
Acetate Oxidation ◽  
Syntrophic Acetate Oxidation

ABSTRACTThis study investigated the process of high-rate, high-temperature methanogenesis to enable very-high-volume loading during anaerobic digestion of waste-activated sludge. Reducing the hydraulic retention time (HRT) from 15 to 20 days in mesophilic digestion down to 3 days was achievable at a thermophilic temperature (55°C) with stable digester performance and methanogenic activity. A volatile solids (VS) destruction efficiency of 33 to 35% was achieved on waste-activated sludge, comparable to that obtained via mesophilic processes with low organic acid levels (<200 mg/liter chemical oxygen demand [COD]). Methane yield (VS basis) was 150 to 180 liters of CH4/kg of VSadded. According to 16S rRNA pyrotag sequencing and fluorescence in situ hybridization (FISH), the methanogenic community was dominated by members of theMethanosarcinaceae, which have a high level of metabolic capability, including acetoclastic and hydrogenotrophic methanogenesis. Loss of function at an HRT of 2 days was accompanied by a loss of the methanogens, according to pyrotag sequencing. The two acetate conversion pathways, namely, acetoclastic methanogenesis and syntrophic acetate oxidation, were quantified by stable carbon isotope ratio mass spectrometry. The results showed that the majority of methane was generated by nonacetoclastic pathways, both in the reactors and in off-line batch tests, confirming that syntrophic acetate oxidation is a key pathway at elevated temperatures. The proportion of methane due to acetate cleavage increased later in the batch, and it is likely that stable oxidation in the continuous reactor was maintained by application of the consistently low retention time.

Performance and energy aspects of single and two phase thermophilic anaerobic digestion of waste activated sludge

2016 ◽  
Vol 86 ◽  
pp. 1324-1331 ◽  
Author(s):  
Wanderli Rogério Moreira Leite ◽  
Marco Gottardo ◽  
Paolo Pavan ◽  
Paulo Belli Filho ◽  
David Bolzonella
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Waste Activated Sludge ◽  
Two Phase ◽  
Thermophilic Anaerobic Digestion ◽  
Energy Aspects

Enhanced two-phase anaerobic digestion of waste-activated sludge by combining magnetite and zero-valent iron

2020 ◽  
Vol 306 ◽  
pp. 123122 ◽  
Author(s):  
Guangyi Zhang ◽  
Yinghao Shi ◽  
Zisheng Zhao ◽  
Xiaowei Wang ◽  
Ming Dou
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Zero Valent Iron ◽  
Waste Activated Sludge ◽  
Two Phase

Syntrophic acetate oxidation in two-phase (acid–methane) anaerobic digesters

2011 ◽  
Vol 64 (9) ◽  
pp. 1812-1820 ◽  
Author(s):  
T. Shimada ◽  
E. Morgenroth ◽  
M. Tandukar ◽  
S. G. Pavlostathis ◽  
A. Smith ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Anaerobic Digestion ◽  
Full Scale ◽  
Laboratory Scale ◽  
Gene Clone ◽  
Rrna Gene ◽  
Acetate Oxidation ◽  
Two Phase ◽  
Anaerobic Digesters ◽  
Syntrophic Acetate Oxidation

The microbial processes involved in two-phase anaerobic digestion were investigated by operating a laboratory-scale acid-phase (AP) reactor and analyzing two full-scale, two-phase anaerobic digesters operated under mesophilic (35 °C) conditions. The digesters received a blend of primary sludge and waste activated sludge (WAS). Methane levels of 20% in the laboratory-scale reactor indicated the presence of methanogenic activity in the AP. A phylogenetic analysis of an archaeal 16S rRNA gene clone library of one of the full-scale AP digesters showed that 82% and 5% of the clones were affiliated with the orders Methanobacteriales and Methanosarcinales, respectively. These results indicate that substantial levels of aceticlastic methanogens (order Methanosarcinales) were not maintained at the low solids retention times and acidic conditions (pH 5.2–5.5) of the AP, and that methanogenesis was carried out by hydrogen-utilizing methanogens of the order Methanobacteriales. Approximately 43, 31, and 9% of the archaeal clones from the methanogenic phase (MP) digester were affiliated with the orders Methanosarcinales, Methanomicrobiales, and Methanobacteriales, respectively. A phylogenetic analysis of a bacterial 16S rRNA gene clone library suggested the presence of acetate-oxidizing bacteria (close relatives of Thermacetogenium phaeum, ‘Syntrophaceticus schinkii,’ and Clostridium ultunense). The high abundance of hydrogen consuming methanogens and the presence of known acetate-oxidizing bacteria suggest that acetate utilization by acetate oxidizing bacteria in syntrophic interaction with hydrogen-utilizing methanogens was an important pathway in the second-stage of the two-phase digestion, which was operated at high ammonium-N concentrations (1.0 and 1.4 g/L). A modified version of the IWA Anaerobic Digestion Model No. 1 (ADM1) with extensions for syntrophic acetate oxidation and weak-acid inhibition adequately described the dynamic profiles of volatile acid production/degradation and methane generation observed in the laboratory-scale AP reactor. The model was validated with historical data from the full-scale digesters.

Characteristics of two-phase mesophilic anaerobic digestion of co-substrates consisting of waste activated sludge and corn silage based on modified ADM1

2019 ◽  
Vol 91 ◽  
pp. 168-178 ◽  
Author(s):  
Haidong Zhou ◽  
Shufeng Lv ◽  
Zhenxi Ying ◽  
Yingying Wang ◽  
Jicheng Liu ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Corn Silage ◽  
Waste Activated Sludge ◽  
Two Phase

Two-Phase Anaerobic Digestion of Waste Activated Sludge:  Effect of an Extreme Thermophilic Prefermentation

2007 ◽  
Vol 46 (21) ◽  
pp. 6650-6655 ◽  
Author(s):  
David Bolzonella ◽  
Paolo Pavan ◽  
Marco Zanette ◽  
Franco Cecchi
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Waste Activated Sludge ◽  
Two Phase
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