Biotin-tagged cis-dichlorido-oxidovanadium(IV) complex for DNA crosslinking and photo-induced apoptotic cytotoxicity

2021 ◽  
Vol 528 ◽  
pp. 120629
Author(s):  
Arun Kumar ◽  
Somarupa Sahoo ◽  
Akanksha Dixit ◽  
Anjali A. Karande ◽  
Akhil R. Chakravarty
Keyword(s):  
Dna Crosslinking

Synthesis of some benzodipyrones, potential photochemical DNA crosslinking agents

1975 ◽  
Vol 12 (2) ◽  
pp. 417-419 ◽  
Author(s):  
John N. Marx ◽  
Pill-Soon Song ◽  
Patrick K. Chui
Keyword(s):  
Dna Crosslinking ◽  
Crosslinking Agents

Abstract 2133: Pre-clinical studies of EC2629, a highly potent FR targeted DNA crosslinking agent

2017 ◽  
Author(s):  
Joseph A. Reddy ◽  
Melissa Nelson ◽  
Christina Dircksen ◽  
Theresa Johnson ◽  
Marilynn Vetzel ◽  
...  
Keyword(s):  
Clinical Studies ◽  
Crosslinking Agent ◽  
Dna Crosslinking

scholarly journals A High-Throughput Screening Strategy to Identify Protein-Protein Interaction Inhibitors That Block the Fanconi Anemia DNA Repair Pathway

2016 ◽  
Vol 21 (6) ◽  
pp. 626-633 ◽  
Author(s):  
Andrew F. Voter ◽  
Kelly A. Manthei ◽  
James L. Keck
Keyword(s):  
Dna Repair ◽  
High Throughput ◽  
Fanconi Anemia ◽  
Fluorescence Polarization ◽  
High Throughput Screening ◽  
Titration Calorimetry ◽  
Common Mechanism ◽  
Physical Interaction ◽  
Repair Pathway ◽  
Dna Crosslinking

Induction of the Fanconi anemia (FA) DNA repair pathway is a common mechanism by which tumors evolve resistance to DNA crosslinking chemotherapies. Proper execution of the FA pathway requires interaction between the FA complementation group M protein (FANCM) and the RecQ-mediated genome instability protein (RMI) complex, and mutations that disrupt FANCM/RMI interactions sensitize cells to DNA crosslinking agents. Inhibitors that block FANCM/RMI complex formation could be useful therapeutics for resensitizing tumors that have acquired chemotherapeutic resistance. To identify such inhibitors, we have developed and validated high-throughput fluorescence polarization and proximity assays that are sensitive to inhibitors that disrupt interactions between the RMI complex and its binding site on FANCM (a peptide referred to as MM2). A pilot screen of 74,807 small molecules was performed using the fluorescence polarization assay. Hits from the primary screen were further tested using the proximity assay, and an orthogonal proximity assay was used to assess inhibitor selectivity. Direct physical interaction between the RMI complex and the most selective inhibitor identified through the screening process was measured by surface plasmon resonance and isothermal titration calorimetry. Observation of direct binding by this small molecule validates the screening protocol.

Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity

2017 ◽  
Vol 174 ◽  
pp. 45-54 ◽  
Author(s):  
Arun Kumar ◽  
Ila Pant ◽  
Akanksha Dixit ◽  
Samya Banerjee ◽  
Bhabatosh Banik ◽  
...  
Keyword(s):  
Dna Crosslinking

scholarly journals DNA crosslinking and cytotoxicity in normal and transformed human cells treated with antitumor nitrosoureas.

1980 ◽  
Vol 77 (1) ◽  
pp. 467-471 ◽  
Author(s):  
L. C. Erickson ◽  
M. O. Bradley ◽  
J. M. Ducore ◽  
R. A. Ewig ◽  
K. W. Kohn
Keyword(s):  
Human Cells ◽  
Dna Crosslinking
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