Dilute-acid pretreatment of barley straw for biological hydrogen production using Caldicellulosiruptor saccharolyticus

2012 ◽  
Vol 37 (16) ◽  
pp. 11727-11734 ◽  
Author(s):  
I.A. Panagiotopoulos ◽  
R.R. Bakker ◽  
T. de Vrije ◽  
P.A.M. Claassen ◽  
E.G. Koukios
2010 ◽  
Vol 150 ◽  
pp. 141-141 ◽  
Author(s):  
Sung Bong Kim ◽  
Ja Hyun Lee ◽  
Sang Jun Lee ◽  
Eun Ji Jang ◽  
Kyeong Keun Oh ◽  
...  

2011 ◽  
Vol 16 (4) ◽  
pp. 725-732 ◽  
Author(s):  
Sung Bong Kim ◽  
Ja Hyun Lee ◽  
Kyeong Keun Oh ◽  
Sang Jun Lee ◽  
Jin Young Lee ◽  
...  

2013 ◽  
Vol 146 ◽  
pp. 444-450 ◽  
Author(s):  
Ming Yang ◽  
Suvi Kuittinen ◽  
Junhua Zhang ◽  
Markku Keinänen ◽  
Ari Pappinen

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong

Abstract Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.


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