scholarly journals A Simple and Cost-Effective Method for Rapid Purification of Alkyl Hydroperoxide Reductase (Ahpc) from Helicobacter pylori

2008 ◽  
Vol 12 ◽  
pp. e207-e208
Author(s):  
T. Mohammadian ◽  
M. Doosti ◽  
M. Paknejad ◽  
F. Siavoshi ◽  
S. Massarrat ◽  
...  
1999 ◽  
Vol 123 (9) ◽  
pp. 778-781 ◽  
Author(s):  
Maher Toulaymat ◽  
Sharon Marconi ◽  
Jane Garb ◽  
Christopher Otis ◽  
Shirin Nash

Abstract Objectives.—To describe the endoscopic biopsy pathology of Helicobacter pylori gastritis, compare bacterial detection by immunohistochemistry using a specific antibody with the Genta stain, and to compare the relative costs of the 2 techniques. Design.—One hundred cases of gastritis identified as positive for H pylori by Genta stain and 100 cases considered negative by the same technique were stained using an anti-H pylori–specific polyclonal antibody. Laboratory reagent and labor costs for the 2 methods were compared. Results.—Chronic active gastritis with lymphoid follicles was significantly associated with H pylori infection (P < .0001). The immunohistochemical method had a sensitivity of 97% and a specificity of 98% compared with the Genta stain, with strong agreement for grading density of organisms (κ = 0.85; P < .001). Reagent costs were similar for both methods, but immunohistochemistry using an autoimmunostainer required less dedicated technical time and hence was less expensive than the Genta stain. Conclusions.—Immunohistochemistry using a specific antibody is an accurate and cost-effective method for H pylori detection in gastric biopsies.


2005 ◽  
Vol 1753 (2) ◽  
pp. 240-246 ◽  
Author(s):  
Elena Papinutto ◽  
Henry J. Windle ◽  
Laura Cendron ◽  
Roberto Battistutta ◽  
Dermot Kelleher ◽  
...  

Helicobacter ◽  
2001 ◽  
Vol 6 (4) ◽  
pp. 274-282 ◽  
Author(s):  
Jing Yan ◽  
Toshiko Kumagai ◽  
Makoto Ohnishi ◽  
Ichiro Ueno ◽  
Hiroyoshi Ota

Vaccine ◽  
2012 ◽  
Vol 30 (26) ◽  
pp. 3876-3884 ◽  
Author(s):  
Avril A. O’Riordan ◽  
Veronica Athie Morales ◽  
Linda Mulligan ◽  
Nazia Faheem ◽  
Henry J. Windle ◽  
...  

2013 ◽  
Vol 195 (23) ◽  
pp. 5396-5401 ◽  
Author(s):  
S. L. Benoit ◽  
K. Bayyareddy ◽  
M. Mahawar ◽  
J. S. Sharp ◽  
R. J. Maier

The choice of cost-effective method of anticorrosive protection of steel structures is an urgent and time consuming task, considering the significant number of protection ways, differing from each other in the complex of technological, physical, chemical and economic characteristics. To reduce the complexity of solving this problem, the author proposes a computational tool that can be considered as a subsystem of computer-aided design and used at the stage of variant and detailed design of steel structures. As a criterion of the effectiveness of the anti-corrosion protection method, the cost of the protective coating during the service life is accepted. The analysis of existing methods of steel protection against corrosion is performed, the possibility of their use for the protection of the most common steel structures is established, as well as the estimated period of effective operation of the coating. The developed computational tool makes it possible to choose the best method of protection of steel structures against corrosion, taking into account the operating conditions of the protected structure and the possibility of using a protective coating.


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