Identification and confirmation of Laribacter hongkongensis bacteremia in a patient with neutropenic sepsis in Malaysia

Objective: Once daily dosing (ODD) aminoglycoside is gaining wide acceptance as an alternative way of dosing. In our setting it is the regimen of choice whenever gentamicin is indicated. The objective of this study was to evaluate the practice of gentamicin ODD in a public hospital in Malaysia. Methods: We conducted a retrospective review of medical records of patients on gentamicin ODD who were admitted to Hospital Melaka during January 2002 until March 2010. All adult patients who were on ODD gentamicin with various level of renal function were included in the study. Patients on gentamicin less than 72 hours and pregnant women were excluded. Results: From 110 patients, 75 (68.2%) were male and 35 (31.8%) were female. Indications for ODD gentamicin included pneumonia, 34 (31.0%) neutropenic sepsis, 27 (24.5%) and sepsis, 11 (10.0%). The mean dose and duration of gentamicin was 3.2 mg/kg/day and 7 days, respectively. Almost all patients were on gentamicin combined with other antibiotics. Clinical cure based on fever resolution was found in 89.1% of patients treated with ODD. Resolution of fever took an average of 48 hours after initiation of therapy. The evaluation for bacteriologic cure could not be performed because of insufficient data on culture and sensitivity. Out of 38 patients with analyzable serum creatinine data, four patients might have developed nephrotoxicity. Conclusion: In our setting, lower dosages of ODD gentamicin when used in combination with other antibiotics seemed to be effective and safe in treating most gram negative infections.

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G57(P) Nurses leading the way: assessing the impact of a nurse-led neutropenic sepsis pathway on achieving the ‘golden hour’ in a paediatric emergency department

10.1136/archdischild-2019-rcpch.57 ◽

2019 ◽

Author(s):

LR Jefferson ◽

H Walker ◽

H Spires ◽

K Jones ◽

W Christian

Keyword(s):

Emergency Department ◽

Golden Hour ◽

Paediatric Emergency Department ◽

Paediatric Emergency ◽

Neutropenic Sepsis ◽

The Impact ◽

The Way

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Characterization of type I-F CRISPR-Cas system in Laribacter hongkongensis isolates from animals, the environment and diarrhea patients

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2021.109153 ◽

2021 ◽

Vol 346 ◽

pp. 109153

Author(s):

Ling Wang ◽

Li Wang ◽

Youzhao Liu ◽

Zhiyun Wang ◽

Qing Chen ◽

...

Keyword(s):

Type I ◽

Laribacter Hongkongensis

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Cloning and Characterization of a Chromosomal Class C β-Lactamase and Its Regulatory Gene in Laribacter hongkongensis

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.49.5.1957-1964.2005 ◽

2005 ◽

Vol 49 (5) ◽

pp. 1957-1964 ◽

Cited By ~ 23

Author(s):

Susanna K. P. Lau ◽

Pak-leung Ho ◽

Maria W. S. Li ◽

Hoi-wah Tsoi ◽

Raymond W. H. Yung ◽

...

Keyword(s):

Genomic Dna ◽

Southern Hybridization ◽

Regulatory Gene ◽

Kinetic Properties ◽

Restriction Fragments ◽

Regulatory Systems ◽

Laribacter Hongkongensis ◽

Consensus Motifs ◽

Class C

ABSTRACT Laribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most β-lactams except the carbapenems. We describe the cloning and characterization of a novel chromosomal class C β-lactamase and its regulatory gene in L. hongkongensis. Two genes, ampC and ampR, were cloned by inserting restriction fragments of genomic DNA from L. hongkongensis strain HLHK5 into pBK-CMV to give the recombinant plasmid pBK-LHK-5. The ampR and ampC genes and their promoters were divergently oriented, with the ampR gene immediately upstream of the ampC gene and an intercistronic Lys-R motif, typical of inducible ampC-ampR regulatory systems. The deduced amino acid sequence of the cloned AmpC β-lactamase (pI 8.1) contained consensus motifs characteristic of class C β-lactamases but had identities no greater than 46% to known class C β-lactamases. The kinetic properties of this AmpC were also compatible with those of a class C β-lactamase. PCR of 20 clinical isolates of L. hongkongensis, including HLHK5, showed the presence of both ampC and ampR genes in all isolates. Southern hybridization suggested that the ampC gene of HLHK5 was chromosomally encoded. Subcloning experiments showed that the expression of the ampC gene of HLHK5 was regulated by its ampR gene, which acts as a repressor. The β-lactamase characterized from strain HLHK5 was named LHK-5 (gene, bla LHK-5) and represents the first example of AmpC β-lactamase in the β subdivision of proteobacteria.

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Development of an ERIC sequence typing scheme for Laribacter hongkongensis, an emerging pathogen associated with community-acquired gastroenteritis and travellers’ diarrhoea

Journal of Medical Microbiology ◽

10.1099/jmm.0.049858-0 ◽

2013 ◽

Vol 62 (5) ◽

pp. 701-707 ◽

Cited By ~ 7

Author(s):

Jia-Li Feng ◽

Jin-Yan Lin ◽

Xiao-Bing Jiang ◽

Ou Zhang ◽

Jiang-Feng Zhu ◽

...

Keyword(s):

Clustering Analysis ◽

Consensus Sequence ◽

Pulsed Field Gel Electrophoresis ◽

Epidemiological Investigation ◽

Enterobacterial Repetitive Intergenic Consensus ◽

Emerging Pathogen ◽

Outbreak Investigations ◽

Laribacter Hongkongensis ◽

Specialized Equipment ◽

Typing Methods

Laribacter hongkongensis is a potential emerging pathogen, associated with community-acquired diarrhoea. For epidemiological purposes, different molecular typing methods, such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing, have been developed for this pathogen. However, these methods require specialized equipment and costly reagents. More importantly, they are labour-intensive and time-consuming, which is not really suitable for foodborne disease outbreak investigations. In this study, we developed a rapid and reliable method using 22-mer primers specific for the enterobacterial repetitive intergenic consensus sequence (ERIC). PFGE was used for comparison, to evaluate this method. A total of 81 isolates of L. hongkongensis were examined: 79 isolates recovered from food of diverse origins and two strains derived from patients with L. hongkongensis-associated infection. Typing patterns and clustering analysis indicated that the 81 L. hongkongensis isolates were grouped into 21 and 13 genotypes by ERIC-PCR and PFGE, respectively. ERIC-PCR was found as reproducible as PFGE. A high percentage (70.4 %) of isolates yielded distinguishable ERIC-PCR patterns, which were concordant with the results from PFGE. These results suggest that ERIC-PCR is valuable for use in the epidemiological investigation of L. hongkongensis.

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