Clinical relevance of virulence genes in Helicobacter pylori isolates recovered from adult dyspeptic patients in Turkey

Background: Helicobacter pylori transmission routes are not entirely elucidated. Since yeasts are postulated to transmit this pathogen, this study aimed to detect and genotype intracellular H. pylori harbored within vaginal yeast cells. Methods: A questionnaire was used to determine risk factors of H. pylori infection. Samples were seeded on Sabouraud Dextrose Agar and horse blood-supplemented Columbia agar. Isolated yeasts were identified using and observed by optical microscopy searching for intra-yeast H. pylori. Total yeast DNA, from one random sample, was extracted to search for H. pylori virulence genes by PCR and bacterial identification by sequencing. Results: 43% of samples contained yeasts, mainly Candida albicans (91%). Microscopy detected bacteria such as bodies and anti-H. pylori antibodies binding particles in 50% of the isolated yeasts. Total DNA extracted showed that 50% of the isolated yeasts were positive for H. pylori 16S rDNA and the sequence showed 99.8% similarity with H. pylori. In total, 32% of H. pylori DNA positive samples were cagA+ vacAs1a vacAm1 dupA−. No relationship was observed between possible H. pylori infection risk factors and vaginal yeasts harboring this bacterium. Conclusion: H. pylori having virulent genotypes were detected within vaginal yeasts constituting a risk for vertical transmission of this pathogen.

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Identification of Helicobacter pylori virulence genes by insertion mutagenesis

European Journal of Gastroenterology & Hepatology ◽

10.1097/00042737-199812000-00121 ◽

1998 ◽

Vol 10 (12) ◽

pp. A33

Author(s):

J. J.E. Bijlsma ◽

C. M.J.E. Vandenbroucke-Grauls ◽

J. G. Kusters

Keyword(s):

Helicobacter Pylori ◽

Virulence Genes ◽

Insertion Mutagenesis

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Peculiarities of polymorphism of Helicobacter pylori virulence genes and IL-1 genes in peptic ulcer of stomach and duodenum, associated with Helicobacter pylori

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2005-0-31-35 ◽

2005 ◽

Vol 4 ◽

pp. 31-35

Author(s):

O. A. Tchernova ◽

E. R. Nasybullina ◽

O. V. Gorshkov ◽

G. F. Shaimardanova ◽

V. M. Tchernov ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Virulence Genes

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Endoscopic Findings and Their Clinical Relevance in 208 Diagnostic Esophagogastroduodenoscopies Made at Guadalajara’s Regional Military Specialty Hospital From September 2018 to March 2020

10.47363/jghr/2020(1)107 ◽

2020 ◽

Vol 1 (1) ◽

pp. 1-5

Author(s):

Viera Dórame Raúl Fernando ◽

◽

Aguirre Sandoval Gloria Estefania ◽

◽

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Clinical Relevance ◽

Digestive Endoscopy ◽

Basic Information ◽

Esophageal Reflux ◽

Endoscopic Findings ◽

Appropriate Treatment ◽

Specialty Hospital ◽

Macroscopic Findings

This technical review provides practical and basic information on the clinical relevance of proximal diagnostic digestive endoscopy in relation to pre- and post-endoscopic diagnoses, anatomopathological diagnosis of biopsies, choice of appropriate treatment and improvement of patients studied. It also highlights the importance of the prevention of esophageal-gastric cancer by previous macroscopic findings and its timely diagnosis, based on 208 reports of Esophagogastroduodenoscopies recorded in the general file database of the Guadalajara Regional Military Specialty Hospital between September 2018 and March 2020. Of the 208 selected reports, the most common pre-endoscopic diagnoses were dyspepsia, gastro-esophageal reflux disease, high digestive tract hemorrhage and dysphagia, which were mostly associated with endoscopic diagnosis of chronic gastropathy being the crucial anatomopathological diagnosis by demonstrating that the bacterium Helicobacter Pylori is primarily responsible for this gastropathy and in initiating

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Prevalence of vacA, cagA, and iceA Virulence Factors of Helicobacter Pylori Isolated from Gastro-duodenal Patients

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2020.14.1.592 ◽

2020 ◽

Vol 14 (1) ◽

pp. 72-79

Author(s):

Ahmed Husham Salman ◽

Aumed Arshad Hawezy

Keyword(s):

Helicobacter Pylori ◽

Virulence Factors ◽

Virulence Genes ◽

Gastric Lymphoma ◽

Signs And Symptoms ◽

Molecular Technique ◽

Peptic Ulcers ◽

Back Ground ◽

H Pylori ◽

Pcr Test

Back ground: Helicobacter pylori are bacteria colonize in the human epithelial cells of the gastrointestinal tract. Its infection causes different diseases, including chronic gastritis, peptic ulcers, gastric lymphoma and adenocarcinoma. H. pylori have many virulence factors attributing in one or more biological functions. Objective: Detecting the prevalence of virulence factor genes vacA, cagA, iceA among strain of H. pylori using molecular technique (PCR). Materials and methods: Sixty patients (27 male and 33 female), aged 18 and above included in the present study who showed signs and symptoms of H. pylori, and undergo endoscopy between period of November 2019 and February 2020. RUT and PCR test done to detect the presence of H. pylori infection, also PCR used to detect the three virulence factors. Results: Result showed that 44 patients, 21 (47.7%) male and 23 (52.3%) female were detected as positive H. pylori infections, among them 13 (29.5%) above 50 years, and 31 (70.4%) were below 50 years. While prevalence of the virulence factors vacA, cagA, and iceA were (100%), (84.1%), and (34.1%) respectively. Conclusion: It can be concluded that the frequency and prevalence of these genes are differed and showed significant differences among them. Also, PCR test is sensitive and accurate for detection of H. pylori virulence genes.

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