Expression analysis of manganese peroxidase gene in white rot fungus L-25 in the presence of amino acids

2009 ◽  
Vol 108 ◽  
pp. S125
Author(s):  
Shinya Fujihara ◽  
Akihiko Sakurai ◽  
Mikio Sakakibara
Keyword(s):  
Amino Acids ◽  
Expression Analysis ◽  
Manganese Peroxidase ◽  
White Rot ◽  
White Rot Fungus ◽  
Peroxidase Gene

Depolymerization of Straw Lignin by Manganese Peroxidase from Nematoloma frowardii is Accompanied by Release of Carbon Dioxide

Holzforschung ◽  
1999 ◽  
Vol 53 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Martin Hofrichter ◽  
Katrin Scheibner ◽  
Friedemann Bublitz ◽  
Ivonne Schneegaß ◽  
Dirk Ziegenhagen ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Manganese Peroxidase ◽  
Gel Permeation Chromatography ◽  
Water Soluble ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Fraction ◽  
Molecular Masses ◽  
Total Lignin ◽  
Soluble Part

SummaryManganese peroxidase preparations (MnP) from the white-rot fungusNematoloma frowardiiwere able to release14CO2directly from14C-labeled milled wheat straw (MWS; total lignin fraction) and milled straw lignin (MSL; dioxane soluble part of MWS). Apart from the formation of14CO2(4–10 %) the treatment of insoluble MWS and MSL with MnP resulted in the formation of water-soluble14C-lignin fragments (lignin solubilization, 14–25%). Analyses with gel permeation chromatography (GPC) demonstrated the formation of lignin fragments with predominant molecular masses around 1 kDa. The extent of MWS mineralization and solubilization was enhanced in the presence of reduced glutathione (GSH) acting as thiol mediator, whereas MSL mineralization was not stimulated by GSH. The principle of direct extracellular mineralization of lignin catalyzed by the MnP system may make a significant contribution to the formation of carbon dioxide in lignincellulose containing habitats.

Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme

2007 ◽  
Vol 41 (3) ◽  
pp. 258-264 ◽  
Author(s):  
Xiaobin Cheng ◽  
Rong Jia ◽  
Pingsheng Li ◽  
Shiqian Tu ◽  
Qin Zhu ◽  
...  
Keyword(s):  
Azo Dyes ◽  
Manganese Peroxidase ◽  
White Rot ◽  
White Rot Fungus
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