Effect of intermittent opening of breathable culture plugs and aeration of headspace on the structure of microbial communities in shake-flask culture

2018 ◽  
Vol 126 (1) ◽  
pp. 96-101 ◽  
Author(s):  
Masato Takahashi ◽  
Hideki Aoyagi
Keyword(s):  
Microbial Communities ◽  
Shake Flask ◽  
Shake Flask Culture ◽  
Flask Culture

scholarly journals Optimization of Medium Ingredients for Keratinolytic Protease Production by Bacillus licheniformis MZK-03 using Statistical Experimental Designs

1970 ◽  
Vol 24 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Mohammad Moniruzzaman ◽  
Alamgir Rahman ◽  
M Mozammel Hoq
Keyword(s):  
Trace Elements ◽  
Bacillus Licheniformis ◽  
Shake Flask ◽  
Culture Medium ◽  
Protease Production ◽  
Shake Flask Culture ◽  
Flask Culture ◽  
Statistical Approaches ◽  
Central Composite ◽  
Keratinolytic Protease

A culture medium was optimized for the production of keratinolytic protease by a newly isolated strain of Bacillus licheniformis MZK-03 in shake-flask culture. Based on the results of preliminary experiments, feather mill, molasses and trace elements were found to be major variables in keratinolytic protease production. The concentrations of these ingredients were optimized by using two statistical approaches, namely Box-Wilson method and central composite design. The optimized culture medium, finally determined by using the statistical approaches, composed of 0.95% feather mill, 0.12% molasses and 1.44% trace elements. The keratinolytic protease production was increased by approximately 2-fold when the strain was grown in the optimized medium (95.2 U/ml) compared to the un-optimized medium (56.05 U/ml). Keywords: Keratinolytic protease, Optimization, Bacillus licheniformis MZK-03, Statistical designsDOI: http://dx.doi.org/10.3329/bjm.v24i1.1238 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 52-56

Study on Screening of Glutathione over-Production Strain and its Fermentation Conditions

2011 ◽  
Vol 396-398 ◽  
pp. 1657-1661
Author(s):  
Jie Gang ◽  
Li Feng Shan ◽  
Hong Yu Bu
Keyword(s):  
Shake Flask ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Shake Flask Culture ◽  
Optimal Conditions ◽  
Flask Culture ◽  
Fermentation Conditions ◽  
Physiological And Biochemical Characteristics ◽  
Physiological And Biochemical ◽  
Production Strain

This study was to screen strains for glutathione over-production. A high GSH-producing strain HSJB1 was isolated from soil.According to the morphological, physiological and biochemical characteristics of cells, this strain was primarily identified as Saccharomyces cerevisiae. The glutathion fermentation conditions of this strain by shake flask culture were studied.The optimal fermentation medium was as follows: glucose 20g/L, (NH4)2SO4 5g/L, peptone 12.5 g/L, KH2PO4 9.0 g/L, MgSO4•7H2O 1.0 g/L, NaCl 0.2 g/L, ZnSO4•H2O 0.01 g/L. The optimal culture conditions were as follows :pH 5，broth quantity 30ml/250ml, temperature 28°C，inoculum 10%. Under the optimal conditions, the glutathione productivity and biomass were 53.60 mg/L and 3.8 g/L, respectively, which were 11.2% and 11.8% higher than those in the original conditions, respectively.

Effect of Coconut Water on the Growth of Gluconacetobacter xylinus Y15

2013 ◽  
Vol 781-784 ◽  
pp. 1736-1740
Author(s):  
Lin Jin Li ◽  
Si Xin Liu ◽  
Cong Fa Li
Keyword(s):  
Shake Flask ◽  
Growth Curves ◽  
Cell Number ◽  
Coconut Water ◽  
Lag Phase ◽  
Shake Flask Culture ◽  
Water Medium ◽  
Gluconacetobacter Xylinus ◽  
Flask Culture ◽  
Water Contents

The effect of coconut water contents, sucrose and ammonium sulfate concentrations, cultivation modes on the growth of Gluconacetobacter xylinus Y15 was investigated. When 25%(V/V) coconut water, 3g/L (NH4)2SO4 was added into the medium without sucrose, and G. xylinus Y15 was cultivated in shake flask culture at 130r/min for 24h, the cell number could reach as high as 4.9×108CFU/mL, while the cell number was only 4.9×104CFU/mL when cultivated in static culture. The growth curves of G. xylinus Y15 in coconut water medium and HS [ medium in shake flask culture were compared. The lag phase of G. xylinus Y15 in coconut water medium was 20h shorter than in HS medium, and the log phase was 20h longer.

Effect of strain and medium variation on mosquito toxin production by Bacillus thuringiensis var. israelensis

1982 ◽  
Vol 28 (9) ◽  
pp. 1089-1092 ◽  
Author(s):  
Robert A. Smith
Keyword(s):  
Bacillus Thuringiensis ◽  
Growth Medium ◽  
Shake Flask ◽  
Culture Medium ◽  
Toxin Production ◽  
Shake Flask Culture ◽  
Mosquito Larvae ◽  
Flask Culture ◽  
Strain Variation ◽  
Spore Count

The effect of strain variation and culture medium on production of toxin lethal to mosquito larvae by Bacillus thuringiensis var. israelensis serotype H-14 was investigated. Shake flask culture of B. thuringiensis H-14 strains showed varied ability to produce toxins lethal to mosquito larvae dependent upon the particular strain and growth medium used. Buffered media demonstrated no better mosquito toxicity than did unbuffered media that ranged in pH from 5.7 to 8.1 at harvest. Although toxin production is associated with sporulation, spore count was generally not proportional to toxin produced for those strains and media evaluated.

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