High cell density suppresses BMP4-induced differentiation of human pluripotent stem cells to produce macroscopic spatial patterning in a unidirectional perfusion culture chamber

2018 ◽  
Vol 126 (3) ◽  
pp. 379-388 ◽  
Author(s):  
Shota Tashiro ◽  
Minh Nguyen Tuyet Le ◽  
Yuta Kusama ◽  
Eri Nakatani ◽  
Mika Suga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Density ◽  
Pluripotent Stem Cells ◽  
High Cell Density ◽  
Perfusion Culture ◽  
Human Pluripotent Stem Cells ◽  
Spatial Patterning ◽  
High Cell ◽  
Culture Chamber ◽  
Induced Differentiation

scholarly journals Auto/paracrine factors and early Wnt inhibition promote cardiomyocyte differentiation from human induced pluripotent stem cells at initial low cell density

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Minh Nguyen Tuyet Le ◽  
Mika Takahi ◽  
Kiyoshi Ohnuma
Keyword(s):  
Stem Cells ◽  
Cell Density ◽  
Pluripotent Stem Cells ◽  
High Cell Density ◽  
High Cell ◽  
Cardiomyocyte Differentiation ◽  
Paracrine Factors ◽  
Induced Pluripotent ◽  
Low Cell Density ◽  
Cardiac Mesoderm

AbstractCardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) have received increasing attention for their clinical use. Many protocols induce cardiomyocytes at an initial high cell density (confluence) to utilize cell density effects as hidden factors for cardiomyocyte differentiation. Previously, we established a protocol to induce hiPSC differentiation into cardiomyocytes using a defined culture medium and an initial low cell density (1% confluence) to minimize the hidden factors. Here, we investigated the key factors promoting cardiomyocyte differentiation at an initial low cell density to clarify the effects of cell density. Co-culture of hiPSCs at an initial low cell density with those at an initial high cell density showed that signals secreted from cells (auto/paracrine factors) and not cell–cell contact signals, played an important role in cardiomyocyte differentiation. Moreover, although cultures with initial low cell density showed higher expression of anti-cardiac mesoderm genes, earlier treatment with a Wnt production inhibitor efficiently suppressed the anti-cardiac mesoderm gene expression and promoted cardiomyocyte differentiation by up to 80% at an initial low cell density. These results suggest that the main effect of cell density on cardiomyocyte differentiation is inhibition of Wnt signaling at the early stage of induction, through auto/paracrine factors.

scholarly journals Spatially Organized Differentiation of Mesenchymal Stem Cells within Biphasic Microparticle-Incorporated High Cell Density Osteochondral Tissues

2015 ◽  
Vol 4 (15) ◽  
pp. 2306-2313 ◽  
Author(s):  
Loran D. Solorio ◽  
Lauren M. Phillips ◽  
Alexandra McMillan ◽  
Christina W. Cheng ◽  
Phuong N. Dang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Density ◽  
High Cell Density ◽  
High Cell

scholarly journals High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome

2018 ◽  
Vol 13 (10) ◽  
pp. 1800036 ◽  
Author(s):  
Leila Zamani ◽  
Magnus Lundqvist ◽  
Ye Zhang ◽  
Magnus Aberg ◽  
Fredrik Edfors ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
High Cell

Production of human-mouse chimeric antibody by high cell density perfusion culture

1989 ◽  
Vol 2 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Yoshiharu Takazawa ◽  
Michiyuki Tokashiki
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
Chimeric Antibody ◽  
High Cell

scholarly journals Alcohol-Induced Delay of Viability Loss in Stationary-Phase Cultures of Escherichia coli

2002 ◽  
Vol 184 (11) ◽  
pp. 2898-2905 ◽  
Author(s):  
Marin Vulić ◽  
Roberto Kolter
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Cell Density ◽  
High Cell Density ◽  
Amino Acid Content ◽  
High Cell ◽  
Induced Differentiation ◽  
Prolonged Incubation ◽  
Viability Loss ◽  
High Amino Acid

ABSTRACT During prolonged incubation in stationary phase Escherichia coli undergoes starvation-induced differentiation, resulting in highly resistant cells. In rich medium with high amino acid content further incubation of cultures at high cell density leads to the generation of a population of cells no longer able to form colonies. The viability loss is due to some component of spent medium, active at high pH and high cell density, and can be prevented either by keeping the pH close to neutrality, by washing off the nonsalt components of the medium, or by keeping the saturating cell density low. Exposure to short-chain n-alcohols within a specific time window in stationary phase also prevents viability loss, in an rpoS-dependent fashion. The development of stress resistance, a hallmark of stationary-phase cells, is affected following alcohol treatment, as is the response to extracellular factors in spent medium. Alcohols seem to block cells in an early phase of starvation-induced differentiation, most likely by interfering with processes important for regulation of σs such as cell density signals and sensing the nutrient content of the medium.

421: High cell density protocol for isolation and expansion of amniotic fluid-derived mesenchymal stem cells

2011 ◽  
Vol 204 (1) ◽  
pp. S170
Author(s):  
Leonardo Gucciardo ◽  
Rik Lories ◽  
Silvia Rusconi ◽  
Philip DeKoninck ◽  
Lieve Coorevits ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Cell Density ◽  
High Cell Density ◽  
High Cell
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