Characterization of a bifunctional aminoacylase/carboxypeptidase from radioresistant bacterium Deinococcus radiodurans R1

2007 ◽  
Vol 128 (2) ◽  
pp. 322-334 ◽  
Author(s):  
L LIN ◽  
M CHEN ◽  
H CHIEN ◽  
S KAN ◽  
C CHEN ◽  
...  
2021 ◽  
Vol 59 (2) ◽  
pp. 186-201
Author(s):  
Immanuel Dhanasingh ◽  
Eunsil Choi ◽  
Jeongeun Lee ◽  
Sung Haeng Lee ◽  
Jihwan Hwang

Chemosphere ◽  
2018 ◽  
Vol 211 ◽  
pp. 1156-1165 ◽  
Author(s):  
Rong Xu ◽  
Kejia Wu ◽  
Huawen Han ◽  
Zhenmin Ling ◽  
Zhengjun Chen ◽  
...  

2015 ◽  
Vol 191 (2) ◽  
pp. 87-99 ◽  
Author(s):  
Aili Sarre ◽  
Mats Ökvist ◽  
Tobias Klar ◽  
David R. Hall ◽  
Arne O. Smalås ◽  
...  

2014 ◽  
Vol 10 (10) ◽  
pp. 2607-2616 ◽  
Author(s):  
Zhongchao Zhao ◽  
Zhengfu Zhou ◽  
Liang Li ◽  
Xianyi Xian ◽  
Xiubin Ke ◽  
...  

Excess copper is toxic to organisms, and therefore, copper homeostasis is important for the limitation of its cellular levels.


2006 ◽  
Vol 51 (24) ◽  
pp. 2993-2999 ◽  
Author(s):  
Qing Zhou ◽  
Xinjue Zhang ◽  
Hong Xu ◽  
Bujin Xu ◽  
Yuejin Hua

2006 ◽  
Vol 188 (2) ◽  
pp. 694-701 ◽  
Author(s):  
Giovanni D'Errico ◽  
Anna Di Salle ◽  
Francesco La Cara ◽  
Mosè Rossi ◽  
Raffaele Cannio

ABSTRACT An open reading frame (draSO) encoding a putative sulfite oxidase (SO) was identified in the sequence of chromosome II of Deinococcus radiodurans; the predicted gene product showed significant amino acid sequence homology to several bacterial and eukaryotic SOs, such as the biochemically and structurally characterized enzyme from Arabidopsis thaliana. Cloning of the Deinococcus SO gene was performed by PCR amplification from the bacterial genomic DNA, and heterologous gene expression of a histidine-tagged polypeptide was obtained in a molybdopterin-overproducing strain of Escherichia coli. The recombinant protein was purified to homogeneity by nickel chelating affinity chromatography, and its main kinetic and chemical physical parameters were determined. Northern blot and enzyme activity analyses indicated that draSO gene expression is constitutive in D. radiodurans and that there is no increase upon exposure to thiosulfate and/or molybdenum(II).


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