Determination of free and liposomal Amphotericin B in human plasma by liquid chromatography–mass spectroscopy with solid phase extraction and protein precipitation techniques

2010 ◽  
Vol 878 (3-4) ◽  
pp. 315-326 ◽  
Author(s):  
Niranjan M. Deshpande ◽  
Manish G. Gangrade ◽  
Maharudra B. Kekare ◽  
Vikas V. Vaidya
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Human Plasma ◽  
Amphotericin B ◽  
Mass Spectroscopy ◽  
Liposomal Amphotericin ◽  
Solid Phase ◽  
Protein Precipitation ◽  
Phase Extraction

scholarly journals A Modified Method for Determination of Lumefantrine in Human Plasma by HPLC-UV and Combination of Protein Precipitation and Solid-Phase Extraction: Application to a Pharmacokinetic Study

2010 ◽  
Vol 5 ◽  
pp. ACI.S4431 ◽  
Author(s):  
Liusheng Huang ◽  
Patricia S. Lizak ◽  
Anura L. Jayewardene ◽  
Florence Marzan ◽  
Ming-Na Tina Lee ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Solid Phase ◽  
Internal Standard ◽  
Pharmacokinetic Interaction ◽  
Gradient Elution ◽  
Protein Precipitation ◽  
Phase Extraction ◽  
Modified Method

An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C8 cartridges. Chromatographic separation was performed on a Zorbax SB-CN HPLC column (3.0 × 150 mm, 3.5 μm) with water/methanol (0.1% TFA) as the mobile phases in a gradient elution mode. Detection was performed using UV/vis detector at λ = 335 nm. The method showed to be linear over a range of 50-10,000 ng/mL with acceptable intra- and inter-day precision and accuracy. The mean recoveries were 88.2% for lumefatrine and 84.5% for the I.S. The internal standard halofantrine is readily available from commercial sources. This method was successfully applied to a pharmacokinetic interaction study between a first-line antimalarial combination (artemether—lumefantrine) and antiretroviral therapy.

scholarly journals Trace determination of chlorpheniramine in human plasma using magnetic dispersive solid-phase extraction based on a graphene oxide/Fe3O4@polythionine nanocomposite combined with high-performance liquid chromatography

RSC Advances ◽  
2017 ◽  
Vol 7 (84) ◽  
pp. 53210-53218 ◽  
Author(s):  
Maryam Ahmadi Daryakenary ◽  
Mohsen Zeeb
Keyword(s):  
High Performance Liquid Chromatography ◽  
Graphene Oxide ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Human Plasma ◽  
High Performance ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction

Graphene oxide/Fe3O4@polythionine (GO/Fe3O4@PTh) nanocomposite was fabricated for magnetic dispersive solid-phase extraction and high-performance liquid chromatography-ultraviolent detection (HPLC) of chlorpheniramine in human plasma.

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