Development and validation of a Sensitive bioanalytical method for the quantitative estimation of Pantoprazole in human plasma samples by LC–MS/MS: Application to bioequivalence study

2010 ◽  
Vol 878 (19) ◽  
pp. 1499-1505 ◽  
Author(s):  
Balasekhara R. Challa ◽  
Sai H.S. Boddu ◽  
Bahlul Z. Awen ◽  
Babu R. Chandu ◽  
Chandrasekhar K. Bannoth ◽  
...  
Keyword(s):  
Human Plasma ◽  
Quantitative Estimation ◽  
Bioequivalence Study ◽  
Plasma Samples ◽  
Bioanalytical Method ◽  
Human Plasma Samples ◽  
Development And Validation

scholarly journals Bioanalytical Method Development and Validation of Memantine in Human Plasma by High Performance Liquid Chromatography with Tandem Mass Spectrometry: Application to Bioequivalence Study

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Ravi Kumar Konda ◽  
B. R. Challa ◽  
Babu Rao Chandu ◽  
Kothapalli B. Chandrasekhar
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Method Development ◽  
Dynamic Range ◽  
Quantitative Estimation ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Bioequivalence Study ◽  
Healthy Human ◽  
Development And Validation

A simple, sensitive, and rapid HPLC-MS/MS method was developed and validated for quantitative estimation of memantine in human plasma. Chromatography was performed on Zorbax SB-C18(4.6×75 mm, 3.5 μm) column. Memantine (ME) and internal standard Memantine-d6(MED6) were extracted by using liquid-liquid extraction and analyzed by LC-ESI-MS/MS using multiple-reaction monitoring (MRM) mode. The assay exhibited a linear dynamic range of 50.00–50000.00 pg/ml for ME in human plasma. This method demonstrated an intra- and interday precision within the range of 2.1–3.7 and 1.4–7.8%, respectively. Further intra- and interday accuracy was within the range of 95.6–99.8 and 95.7–99.1% correspondingly. The mean recovery of ME and MED6 was86.07±6.87and80.31±5.70%, respectively. The described method was successfully employed in bioequivalence study of ME in Indian male healthy human volunteers under fasting conditions.

Development and Validation of a Chromatographic Ultraviolet Method for the Quantification of Isavuconazole in Human Plasma Samples

2018 ◽  
Vol 40 (4) ◽  
pp. 512-514 ◽  
Author(s):  
Valeria Cozzi ◽  
Sara Baldelli ◽  
Simone Castoldi ◽  
Emilio Clementi ◽  
Dario Cattaneo
Keyword(s):  
Human Plasma ◽  
Plasma Samples ◽  
Human Plasma Samples ◽  
Development And Validation
Sign in / Sign up

Export Citation Format

Share Document