Direct analysis – no sample preparation – of bioavailable cortisol in human plasma by weak affinity chromatography (WAC)

2017 ◽  
Vol 1061-1062 ◽  
pp. 438-444 ◽  
Author(s):  
Sten Ohlson ◽  
Jagjit Kaur ◽  
Manfred Raida ◽  
Ulf Niss ◽  
Tim Bengala ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Affinity Chromatography ◽  
Human Plasma ◽  
Direct Analysis ◽  
No Sample Preparation

scholarly journals The Application of NMR Spectroscopy and Chemometrics in Authentication of Spices

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 382
Author(s):  
Barbara Pacholczyk-Sienicka ◽  
Grzegorz Ciepielowski ◽  
Łukasz Albrecht
Keyword(s):  
Quality Assurance ◽  
Nmr Spectroscopy ◽  
Sample Preparation ◽  
Bioactive Compounds ◽  
Analytical Methods ◽  
Structural Information ◽  
Chemometric Analysis ◽  
Spectroscopic Methods ◽  
Regular Consumption ◽  
No Sample Preparation

Spices and herbs are among the most commonly adulterated food types. This is because spices are widely used to process food. Spices not only enhance the flavor and taste of food, but they are also sources of numerous bioactive compounds that are significantly beneficial for health. The healing effects of spices are connected with their antimicrobial, anti-inflammatory and carminative properties. However, regular consumption of adulterated spices may cause fatal damage to our system because adulterants in most cases are unhealthy. For that reason, the appropriate analytical methods are necessary for quality assurance and to ensure the authenticity of spices. Spectroscopic methods are gaining interest as they are fast, require little or no sample preparation, and provide rich structural information. This review provides an overview of the application of NMR spectroscopy combined with chemometric analysis to determine the quality and adulteration of spices.

scholarly journals Green Approaches to Sample Preparation Based on Extraction Techniques

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1719 ◽  
Author(s):  
Alshymaa Aly ◽  
Tadeusz Górecki
Keyword(s):  
Sample Preparation ◽  
Analytical Method ◽  
Bioanalytical Chemistry ◽  
Complete Elimination ◽  
Extraction Techniques ◽  
Green Extraction ◽  
Crucial Step ◽  
Recent Developments ◽  
Environmentally Friendly Approach ◽  
No Sample Preparation

Preparing a sample for analysis is a crucial step of many analytical procedures. The goal of sample preparation is to provide a representative, homogenous sample that is free of interferences and compatible with the intended analytical method. Green approaches to sample preparation require that the consumption of hazardous organic solvents and energy be minimized or even eliminated in the analytical process. While no sample preparation is clearly the most environmentally friendly approach, complete elimination of this step is not always practical. In such cases, the extraction techniques which use low amounts of solvents or no solvents are considered ideal alternatives. This paper presents an overview of green extraction procedures and sample preparation methodologies, briefly introduces their theoretical principles, and describes the recent developments in food, pharmaceutical, environmental and bioanalytical chemistry applications.

Analysis for lead in undiluted whole blood by tantalum ribbon atomic absorption spectrophotometry.

1978 ◽  
Vol 24 (7) ◽  
pp. 1182-1185 ◽  
Author(s):  
B L Therrell ◽  
J M Drosche ◽  
T W Dziuk
Keyword(s):  
Sample Preparation ◽  
Atomic Absorption ◽  
Whole Blood ◽  
Extended Period ◽  
Atomic Absorption Spectrophotometry ◽  
Single Beam ◽  
Absorption Spectrophotometry ◽  
Primary Screening ◽  
Beam System ◽  
No Sample Preparation

Abstract We describe a modified tantalum ribbon atomic absorption procedure for determining lead in undiluted whole blood. An instrumentation Laboratory (I.L.) Model 151 atomic absorption spectrophotometer equipped with an I.L. Model 355 Flameless Sampler was used. The Flameless Sampler was slightly modified to include three-cycle operation instead of the normal two cycles. This modified single-beam system, equipped with background correction, allows 5-microliter specimens of whole blood to be quickly and accurately analyzed. No sample preparation other than vortex mixing is involved and method reliability has been demonstrated during an extended period of successful participation in proficiency testing studies conducted by the Center for Disease Control. This tantalum ribbon methodology has further been demonstrated to be effective both as a primary screening procedure and as a confirmatory procedure, when coupled with erythrocyte protoporphyrin determinations, in screening over 300 000 clients during a three-year period of use in the Early and Periodic Screening, Diagnosis and Treatment (EPSDT) Program in Texas.

Distinction of Plasma Inhibitor of Activator-Induced Clot Lysis from Antiplasmins

1975 ◽  
Author(s):  
N. Aoki ◽  
M. Matsuda ◽  
M. Moroi ◽  
N. Yoshida
Keyword(s):  
Affinity Chromatography ◽  
Human Plasma ◽  
Gel Filtration ◽  
Plasminogen Activators ◽  
Inhibitory Effect ◽  
Clot Lysis ◽  
Plasminogen Activation ◽  
Lysis Time ◽  
Α2 Macroglobulin ◽  
Clot Lysis Time

A fraction of human plasma prolongs the activator-induced clot lysis time and inhibits plasminogen activation by the plasminogen activators derived from various sources (urine and tissues). This fraction, designated as antiactivator fraction, was separatid from antiplasmin fractions (α2-macroglobulin and α1-antitrypsin) by gel filtration and affinity chromatography on Sepharose coupled with IgG of antiserum to α1-antitrypsin. Anti-activator fraction thus obtained exerted little antiplasmin activity but inhibited strongly activator-induced clot lysis.Inhibitory effect of plasma on urokinase-induced clot lysis (antiactivator activity) was assayed in various diseases and compared with antiplasmin activity. No correlation was found between the two activities, and it was concluded that the two activities are independent and are ascribed to two different entities.

A Highly Purified Antithrombin III Concentrate Prepared From Human Plasma Fraction IV-1 by Affinity Chromatography

Vox Sanguinis ◽  
1994 ◽  
Vol 67 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Wytold R. Lebing ◽  
David J. Hammond ◽  
James E. Wydick ◽  
George A. Baumbach
Keyword(s):  
Affinity Chromatography ◽  
Human Plasma ◽  
Antithrombin Iii ◽  
Plasma Fraction
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