In ChinaAtractylodis Rhizomais widely used for the treatment of rheumatic diseases and digestive disorders. Stir-frying with wheat bran is the most common processing method. In order to clarify the influence of processing on pharmacological properties ofAtractylodis Rhizoma, an investigation was carried out to compare the pharmacokinetics and tissue distribution of typical constituent after oral administration of rawAtractylodis Rhizomaand processed ones. A simple, rapid, and sensitive high performance liquid chromatography with UV detection was developed and validated for the determination of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate in rat plasma. A chromatography was carried out on Diamonsil C18(250 × 4.6 mm; 5 μm) analytical column, using a mobile phase which consisted of acetonitrile and 0.1% phosphoric acid water (60 : 40, v/v) at a flow rate of 1.0 mL·min−1. The wavelength was set at 336 nm. The LLOQ of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate was 0.00143 μg·mL−1. Both accuracy and precision were satisfactory. The pharmacokinetic results showed that theTmaxwas 1 hour in advance and theCmaxwas increased after processing. Tissue distribution showed that the highest level was in spleen. And the concentrations in the spleen were increased after stir-frying with bran.
Development and Validation of a Sensitive UFLC–MS/MS Method for Quantification of Quercitrin in Plasma: Application to a Tissue Distribution Study
