Bio-analytical liquid chromatographic-based method with a mixed mode online solid phase extraction for drug monitoring of fluconazole in human serum

2021 ◽  
pp. 123045
Author(s):  
Walaa Zarad ◽  
Heba El-Gendy ◽  
Lamyaa Bazan ◽  
Ahmed Ali ◽  
Yasmine Aboulella ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Serum ◽  
Drug Monitoring ◽  
Mixed Mode ◽  
Solid Phase ◽  
Phase Extraction ◽  
Online Solid Phase Extraction ◽  
Liquid Chromatographic

scholarly journals Application of a Mixed-Mode Solid-Phase Extraction and Cleanup Procedure for LC/MS Determination of Thiabendazole and Carbendazim in Apple Juice

2001 ◽  
Vol 84 (5) ◽  
pp. 1608-1614 ◽  
Author(s):  
Michael S Young ◽  
Michael F Early ◽  
Claude R Mallet ◽  
Jim Krol
Keyword(s):  
Solid Phase Extraction ◽  
Mixed Mode ◽  
Apple Juice ◽  
Solid Phase ◽  
Ionization Mass ◽  
Phase Extraction ◽  
Cleanup Procedure ◽  
Sample Preparation Procedure ◽  
Liquid Chromatographic

Abstract Recently, a mixed-mode solid-phase extraction (SPE) procedure was developed for rapid extraction and cleanup for determination of the fungicides thiabendazole and carbendazim in various fruit juices. This paper reports the application of that sample preparation procedure to the liquid chromatographic/mass spectrometric determination of these fungicides in apple juice with detection by positive electrospray ionization mass spectrometry (ESI/MS). Response was linear for sample concentrations from 2 to 500 μg/L (ppb). Recoveries averaged 74% (9% RSD) for carbendazim and 93% (9% RSD) for thiabendazole. After SPE cleanup, no matrix supression was observed for the ESI+ response for either compound studied. The method was applied to the analysis of incurred residues in 4 store-bought apple juices; carbendazim levels ranged from 10 to 70 μg/L and thiabendazole levels ranged from less than 2 to 130 μg/L.

scholarly journals Mixed-Mode Solid-Phase Extraction and Cleanup Procedures for the Liquid Chromatographic Determination of Thiabendazole and Carbendazim in Fruit Juices

2001 ◽  
Vol 84 (2) ◽  
pp. 556-562 ◽  
Author(s):  
Michael S Young ◽  
Dorothy J Phillips ◽  
Pamela C Iraneta ◽  
Jim Krol
Keyword(s):  
Solid Phase Extraction ◽  
Cation Exchange ◽  
Mixed Mode ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Grape Juices ◽  
Liquid Chromatographic

Abstract Solid-phase extraction (SPE) procedures were developed for rapid cleanup and determination of thiabendazole and carbendazim in orange, apple, and grape juices. Samples were prepared by using an SPE cartridge containing a mixed-mode sorbent with both reversed-phase and strong cation-exchange chemistries. Analysis was by liquid chromatography with photodiode-array UV detection. Orange juice was analyzed by mixed-mode cation-exchange extraction with reversed-phase cleanup; the other juices were analyzed by reversed-phase extraction with cation-exchange cleanup. Recoveries >80% for carbendazim and >90% for thiabendazole. Quantitation limits were 20 μg/L for both analytes.

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