Effect of glycerol with sodium chloride on the Krafft point of sodium dodecyl sulfate using surface tension

2019 ◽  
Vol 538 ◽  
pp. 75-82 ◽  
Author(s):  
Haris Khan ◽  
John M Seddon ◽  
Robert V Law ◽  
Nicholas J Brooks ◽  
Eric Robles ◽  
...  
Keyword(s):  
Surface Tension ◽  
Sodium Chloride ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Krafft Point ◽  
Dodecyl Sulfate

Hepatic and lipoprotein lipases selectively assayed in postheparin plasma

1993 ◽  
Vol 39 (2) ◽  
pp. 218-223 ◽  
Author(s):  
A D Henderson ◽  
W Richmond ◽  
R S Elkeles
Keyword(s):  
Sodium Chloride ◽  
Sodium Dodecyl Sulfate ◽  
Lipoprotein Lipase ◽  
Sex Difference ◽  
Hepatic Lipase ◽  
Sodium Dodecyl ◽  
Plasma Samples ◽  
Dodecyl Sulfate ◽  
Normal Adults

Abstract Sensitive, reliable procedures are reported for the selective assay of lipoprotein lipase (LPL) and hepatic lipase (HL) in postheparin plasma samples. LPL is inhibited in the HL assay by inclusion of 0.76 mol/L sodium chloride in the substrate. In the LPL assay, specificity is attained by pretreating the sample with sodium dodecyl sulfate, which selectively denatures HL. This LPL method was validated by direct comparison with a procedure in which HL is inactivated by an antiserum to human HL. We used the described assays to quantify LPL and HL activity in 32 normal adults, demonstrating a clear sex difference for both enzymes. On average, the men displayed higher HL and lower LPL activities than did the women.

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