Detection of viral DNA by isothermal NASBA amplification and chemiluminescence gene probe hybridization assay in a microfluidic cartridge

2015 ◽  
Vol 70 ◽  
pp. S91-S92 ◽  
Author(s):  
F. Bonvicini ◽  
M. Mirasoli ◽  
M. Zangheri ◽  
A. Nascetti ◽  
G. De Cesare ◽  
...  
Keyword(s):  
Hybridization Assay ◽  
Gene Probe ◽  
Viral Dna ◽  
Probe Hybridization

Detection of enteric adenoviruses in south African waters using gene probes

1995 ◽  
Vol 31 (5-6) ◽  
pp. 345-350 ◽  
Author(s):  
B. Genthe ◽  
M. Gericke ◽  
B. Bateman ◽  
N. Mjoli ◽  
R. Kfir
Keyword(s):  
South African ◽  
Water Samples ◽  
Cellulose Fibre ◽  
Gene Probe ◽  
Viral Dna ◽  
Virus Particles ◽  
Gene Probes ◽  
Probe Hybridization ◽  
Enteric Adenoviruses ◽  
Effect Of Chlorine

Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultrafiltration and analysed directly for the presence of enteric adenoviruses. Three pretreatment techniques, namely sephadex columns, cellulose fibre and GenecleanTM were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed. Cellulose fibre and GenecleanTM were found to successfully remove inhibitory substances from concentrated raw waters. Viral DNA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.

Value of Cellobiose–Polymyxin B–Colistin Agar for Isolation of Vibrio vulnificus from Oysters

1995 ◽  
Vol 58 (4) ◽  
pp. 439-440 ◽  
Author(s):  
YI SUN ◽  
JAMES D. OLIVER
Keyword(s):  
Vibrio Vulnificus ◽  
Polymyxin B ◽  
Colony Morphology ◽  
Gene Probe ◽  
Probe Hybridization

Twenty-eight samples, comprising a total of 224 oysters, were examined for the presence of Vibrio vulnificus. Oyster homogenates were plated onto cellobiose—polymyxin B–colistin (CPC) agar or V. vulnificus enumeration (VVE) agar, with subsequent hybridization with a gene probe specific for this pathogen. Of over 3,500 cellobiose-positive colonies initially tested from CPC agar, 28.7% could be identified as V. vulnificus on the basis of probe hybridization. Of the 19,000 colonies developing on VVE agar, only 2.8% were identified as this species. When in subsequent CPC agar studies colony morphology as well as color was considered, 81.6% of over 1,000 colonies probed proved to be V. vulnificus. We conclude that CPC agar is highly selective for this pathogen, and may be effectively employed in monitoring studies to determine levels of this bacterium in molluscan shellfish.

Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay

1989 ◽  
Vol 14 (3) ◽  
pp. 279-287 ◽  
Author(s):  
Kakoli Banerjee ◽  
Gita Sharma ◽  
S. Upadhyay ◽  
B. S. Anand ◽  
G. S. Raju ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Dna Probe ◽  
Hybridization Assay ◽  
Blood Samples ◽  
B Virus ◽  
Probe Hybridization

scholarly journals Detection of Listeria in Foods by Using a DNA-probe Hybridization Assay Kit

1991 ◽  
Vol 44 (8) ◽  
pp. 851-855
Author(s):  
AKIKO NAKAMA ◽  
YATARO KOKUBO ◽  
TAKASHI IIDA ◽  
FUJIO UMEKI ◽  
TSUTOMU MARUYAMA
Keyword(s):  
Dna Probe ◽  
Hybridization Assay ◽  
Probe Hybridization

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

The infection and viral DNA replication ofBocavirusminute virus of canine in permissive cells and non-permissive cells

2010 ◽  
Vol 34 (8) ◽  
pp. S60-S60
Author(s):  
Yuning Sun ◽  
Fang Li ◽  
Jianming Qiu ◽  
Xiaohong Lu
Keyword(s):  
Dna Replication ◽  
Viral Dna ◽  
Viral Dna Replication
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