Conjugation and Characterization of Latex Particles with Toxoplasma gondii–specific Immunoglobulin Y Antibodies for Diagnostic Aim and Evaluation Efficiency in In Vitro Culture

2020 ◽  
Vol 92 ◽  
pp. 103145
Author(s):  
Rabia Cakir-Koc ◽  
Yasemin Budama-Kilinc ◽  
Eslin Ustun ◽  
Cahit Babur
Keyword(s):  
Toxoplasma Gondii ◽  
In Vitro Culture ◽  
Immunoglobulin Y ◽  
Latex Particles ◽  
Specific Immunoglobulin

In vitro culture and characterization of gene targeted mouse endothelium

2001 ◽  
Vol 173 (1) ◽  
pp. 151-157 ◽  
Author(s):  
C. G. Kevil ◽  
D. C. Bullard
Keyword(s):  
In Vitro Culture

Isolation and characterization of toxoplasma exo-antigens from In vitro culture in MRC5 and vero cells

1987 ◽  
Vol 17 (3) ◽  
pp. 829-834 ◽  
Author(s):  
Bernabe Chumpitazi ◽  
Pierre Ambroise-Thomas ◽  
Maryvonne Cagnard ◽  
Jean M. Autheman
Keyword(s):  
In Vitro Culture ◽  
Vero Cells ◽  
Isolation And Characterization

In vitro culture and cloning of Toxoplasma gondii in a newly established cell line derived from TG180

1991 ◽  
Vol 21 (1) ◽  
pp. 129-132 ◽  
Author(s):  
A. Couatarmanach ◽  
P. Andre ◽  
D. Le Minous ◽  
L. Martin ◽  
R. Robert ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
In Vitro Culture ◽  
Cell Line ◽  
Established Cell Line ◽  
Established Cell

scholarly journals MYST Family Histone Acetyltransferases in the Protozoan Parasite Toxoplasma gondii

2005 ◽  
Vol 4 (12) ◽  
pp. 2057-2065 ◽  
Author(s):  
Aaron T. Smith ◽  
Samantha D. Tucker-Samaras ◽  
Alan H. Fairlamb ◽  
William J. Sullivan
Keyword(s):  
Toxoplasma Gondii ◽  
Catalytic Domain ◽  
Regulation Of Gene Expression ◽  
Protozoan Parasite ◽  
Opportunistic Pathogen ◽  
Covalent Modification ◽  
Histone H4 ◽  
Genomic Locus

ABSTRACT The restructuring of chromatin precedes tightly regulated events such as DNA transcription, replication, and repair. One type of chromatin remodeling involves the covalent modification of nucleosomes by histone acetyltransferase (HAT) complexes. The observation that apicidin exerts antiprotozoal activity by targeting a histone deacetyltransferase has prompted our search for more components of the histone modifying machinery in parasitic protozoa. We have previously identified GNAT family HATs in the opportunistic pathogen Toxoplasma gondii and now describe the first MYST (named for members MOZ, Ybf2/Sas3, Sas2, and Tip60) family HATs in apicomplexa (TgMYST-A and -B). The TgMYST-A genomic locus is singular and generates a ∼3.5-kb transcript that can encode two proteins of 411 or 471 amino acids. TgMYST-B mRNA is ∼7.0 kb and encodes a second MYST homologue. In addition to the canonical MYST HAT catalytic domain, both TgMYST-A and -B possess an atypical C2HC zinc finger and a chromodomain. Recombinant TgMYST-A exhibits a predilection to acetylate histone H4 in vitro at lysines 5, 8, 12, and 16. Antibody generated to TgMYST-A reveals that both the long and short (predominant) versions are present in the nucleus and are also plentiful in the cytoplasm. Moreover, both TgMYST-A forms are far more abundant in rapidly replicating parasites (tachyzoites) than encysted parasites (bradyzoites). A bioinformatics survey of the Toxoplasma genome reveals numerous homologues known to operate in native MYST complexes. The characterization of TgMYST HATs represents another important step toward understanding the regulation of gene expression in pathogenic protozoa and provides evolutionary insight into how these processes operate in eukaryotic cells in general.

scholarly journals Obtaining, purification and characterization of specific immunoglobulin Y with diagnostic and prophylactic aims

2016 ◽  
Vol 18 (1) ◽  
pp. 49-58
Author(s):  
Ricardo Enrique Toso ◽  
◽  
Barbara Daniela Neher ◽  
Nicolás José María Alvarez Rubianes ◽  
Mirta Susana Toribio ◽  
...  
Keyword(s):  
Immunoglobulin Y ◽  
Purification And Characterization ◽  
Specific Immunoglobulin

scholarly journals The Toxoplasma gondii Cyst Wall Interactome

mBio ◽  
2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Vincent Tu ◽  
Tadakimi Tomita ◽  
Tatsuki Sugi ◽  
Joshua Mayoral ◽  
Bing Han ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Protein Interactions ◽  
Cyst Wall ◽  
Affinity Purification ◽  
Dense Granule ◽  
Hypothetical Proteins ◽  
Content Type

ABSTRACT A characteristic of the latent cyst stage of Toxoplasma gondii is a thick cyst wall that forms underneath the membrane of the bradyzoite vacuole. Previously, our laboratory group published a proteomic analysis of purified in vitro cyst wall fragments that identified an inventory of cyst wall components. To further refine our understanding of the composition of the cyst wall, several cyst wall proteins were tagged with a promiscuous biotin ligase (BirA*), and their interacting partners were screened by streptavidin affinity purification. Within the cyst wall pulldowns, previously described cyst wall proteins, dense granule proteins, and uncharacterized hypothetical proteins were identified. Several of the newly identified hypothetical proteins were validated to be novel components of the cyst wall and tagged with BirA* to expand the model of the cyst wall interactome. Community detection of the cyst wall interactome model revealed three distinct clusters: a dense granule, a cyst matrix, and a cyst wall cluster. Characterization of several of the identified cyst wall proteins using genetic strategies revealed that MCP3 affects in vivo cyst sizes. This study provides a model of the potential protein interactions within the cyst wall and the groundwork to understand cyst wall formation. IMPORTANCE A model of the cyst wall interactome was constructed using proteins identified through BioID. The proteins within this cyst wall interactome model encompass several proteins identified in a prior characterization of the cyst wall proteome. This model provides a more comprehensive understanding of the composition of the cyst wall and may lead to insights on how the cyst wall is formed.

Sign in / Sign up

Export Citation Format

Share Document