Abstract
Purpose To investigate the effects of capsaicin on migration and invasion of esophageal squamous cell carcinoma and the roles of claudin-3 and epithelial-mesenchymal transition (EMT).Methods Cldn3 expression was detected in paired cancerous and adjacent normal esophageal tissues by IHC. The association of Cldn3 with clinical data was analyzed. The migration and invasion of ESCC cells were investigated by exposure of capsaicin. The migration and invasion of ESCC cells with Cldn3 silencing or Cldn3 overexpression were also evaluated. The effects of capsaicin on lung metastasis of ESCC with or without Cldn3 silencing were evaluated.Results: The positive expression of Cldn3 was associated with better prognosis of ESCC patients. Capsaicin attenuated migration and invasion of ESCC and inhibited EMT in a dose-dependent manner. Cldn3 silencing enhanced migration and invasion and EMT in ESCC cells. Cldn3 overexpression inhibited migration and invasion and EMT, similar to exposure of capsaicin. Cldn3 silencing also attenuated inhibition of migration and invasion and EMT by exposure of capsaicin. Cldn3 silencing attenuated inhibition of lung metastasis of ESCC by exposure of capsaicin in vivo.Conclusion The positive expression of Cldn3 showed a better prognosis of ESCC, which was a potentially prognostic indicator. Capsaicin inhibited migration and invasion of ESCC through up-regulation of Cldn3 and inhibition of EMT.
Capsaicin inhibits migration and invasion via inhibiting epithelial-mesenchymal transition in esophageal squamous cell carcinoma by up-regulation of claudin-3 expression
