Using a vanA polymerase chain reaction to detect environmental contamination during a vancomycin-resistant enterococci outbreak

2017 ◽  
Vol 97 (4) ◽  
pp. 419-421 ◽  
Author(s):  
M.I. Garvey ◽  
C.W. Bradley ◽  
A. Casey ◽  
V. Clewer ◽  
E. Holden
Keyword(s):  
Polymerase Chain Reaction ◽  
Environmental Contamination ◽  
Chain Reaction ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Polymerase Chain

scholarly journals Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

2011 ◽  
Vol 44 (5) ◽  
pp. 631-632
Author(s):  
Vlademir Cantarelli ◽  
Bianca Cavalcante ◽  
Diogo André Pilger ◽  
Fabiana Souza ◽  
Cícero Gomes Dias ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Surveillance Program ◽  
Southern Brazil ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Polymerase Chain ◽  
Low Sensitivity

INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. CONCLUSIONS: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.

scholarly journals Environmental Contamination of SARS-CoV-2 in a Non-Healthcare Setting

2020 ◽  
Vol 18 (1) ◽  
pp. 117
Author(s):  
Judith Chui Ching Wong ◽  
Hapuarachchige Chanditha Hapuarachchi ◽  
Sathish Arivalan ◽  
Wei Ping Tien ◽  
Carmen Koo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Environmental Contamination ◽  
Healthcare Setting ◽  
Chain Reaction ◽  
Air Samples ◽  
Surface Disinfection ◽  
Healthcare Settings ◽  
Virus Contamination ◽  
Polymerase Chain ◽  
Cleaning And Disinfection

Fomite-mediated transmission has been identified as a possible route for the spread of COVID-19 disease caused by SARS-CoV-2. In healthcare settings, environmental contamination by SARS-CoV-2 has been found in patients’ rooms and toilets. Here, we investigated environmental presence of SARS-CoV-2 in non-healthcare settings and assessed the efficacy of cleaning and disinfection in removing virus contamination. A total of 428 environmental swabs and six air samples was taken from accommodation rooms, toilets and elevators that have been used by COVID-19 cases. By using a reverse transcription polymerase chain reaction assay, we detected two SARS-CoV-2 RNA positive samples in a room where a COVID-19 patient stayed prior to diagnosis. The present study highlights the risk of fomite-mediated transmission in non-healthcare settings and the importance of surface disinfection in spaces occupied by cases. Of note, neither air-borne transmission nor surface contamination of elevators, which were transiently exposed to infected individuals, was evident among samples analyzed.

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