scholarly journals Faster and economical screening for vancomycin-resistant enterococci by sequential use of chromogenic agar and real-time polymerase chain reaction

2017 ◽  
Vol 50 (4) ◽  
pp. 448-453 ◽  
Author(s):  
Thean Yen Tan ◽  
Boran Jiang ◽  
Lily Siew Yong Ng
2011 ◽  
Vol 44 (5) ◽  
pp. 631-632
Author(s):  
Vlademir Cantarelli ◽  
Bianca Cavalcante ◽  
Diogo André Pilger ◽  
Fabiana Souza ◽  
Cícero Gomes Dias ◽  
...  

INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. CONCLUSIONS: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.


2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske

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