scholarly journals Comparison of direct cultivation on a selective solid medium, polymerase chain reaction from an enrichment broth, and the BD GeneOhm™ VanR Assay for identification of vancomycin-resistant enterococci in screening specimens

2011 ◽  
Vol 70 (4) ◽  
pp. 512-521 ◽  
Author(s):  
Guido Werner ◽  
Annerose Serr ◽  
Sabine Schütt ◽  
Christian Schneider ◽  
Ingo Klare ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Solid Medium ◽  
Chain Reaction ◽  
Enrichment Broth ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Polymerase Chain

scholarly journals Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat

2012 ◽  
Vol 32 (1) ◽  
pp. 201-208 ◽  
Author(s):  
Carla Bertechini Faria ◽  
Giovana Caputo Almeida-Ferreira ◽  
Karina Bertechine Gagliardi ◽  
Tatiane Cristina Albuquerque Alves ◽  
Dauri José Tessmann ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Fusarium Graminearum ◽  
Genomic Dna ◽  
Solid Medium ◽  
Food Contamination ◽  
Chain Reaction ◽  
Specific Pcr ◽  
Culture Characteristics ◽  
Mycotoxigenic Fungi ◽  
Polymerase Chain

The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum.

scholarly journals Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

2011 ◽  
Vol 44 (5) ◽  
pp. 631-632
Author(s):  
Vlademir Cantarelli ◽  
Bianca Cavalcante ◽  
Diogo André Pilger ◽  
Fabiana Souza ◽  
Cícero Gomes Dias ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Surveillance Program ◽  
Southern Brazil ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Polymerase Chain ◽  
Low Sensitivity

INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. CONCLUSIONS: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.

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