Isolation, identification and characterization of marine bacteria exhibiting complementary enantioselective epoxide hydrolase activity for preparing chiral chlorinated styrene oxide derivatives

2015 ◽  
Vol 28 ◽  
pp. 225-228 ◽  
Author(s):  
Jung-Hee Woo ◽  
Kyoung-Myoung Kang ◽  
Tae-Hyung Kwon ◽  
Nyun-Ho Park ◽  
Eun Yeol Lee
2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Hae-Seon Kim ◽  
Sun Ho Cha ◽  
Ho Young Suk ◽  
Nyun-Ho Park ◽  
Jung-Hee Woo

ABSTRACT Sphingorhabdus sp. YGSMI21 is a novel strain exhibiting high enantioselective hydrolysis activity for styrene oxide. Here, we present its complete genome sequence, consisting of one circular chromosome (3.86 Mb) and one plasmid (0.196 Mb).


2017 ◽  
Vol 15 (1) ◽  
pp. 15-20 ◽  
Author(s):  
YOPI YOPI ◽  
APRIDAH CAMELIAWATI DJOHAN ◽  
NANIK RAHMANI ◽  
ALIFAH MAFATIKHUL JANNAH

Yopi, Djohan AC, Rahmani N, Jannah AM. 2017. Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp. Biofarmasi (Rumphius J Nat Prod Biochem) 15: 15-20. Isolation, identification, and characterization of mannanase, xylanase and cellulase producing indigenous marine bacteria have been conducted from total 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified as Bacillus subtilis (M8), Bacillus tequilensis (X4) and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50oC, 5.5;70oC and 8;50oC, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has potential to hydrolyzed mannan and xylan for producing oligosaccharides with size around tri-hexasaccharides as a main product.


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