Rapid detection and counting of viable beer-spoilage lactic acid bacteria using a monoclonal chemiluminescence enzyme immunoassay and a CCD camera

2005 ◽  
Vol 303 (1-2) ◽  
pp. 92-104 ◽  
Author(s):  
Carmen March ◽  
Juan J. Manclús ◽  
Antonio Abad ◽  
Alfonso Navarro ◽  
Angel Montoya
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Ccd Camera ◽  
Detection And Counting ◽  
Beer Spoilage ◽  
Chemiluminescence Enzyme Immunoassay

Rapid detection and identification of beer-spoilage lactic acid bacteria by microcolony method

2009 ◽  
Vol 108 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Shizuka Asano ◽  
Kazumaru Iijima ◽  
Koji Suzuki ◽  
Yasuo Motoyama ◽  
Tomoo Ogata ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rapid Detection ◽  
Detection And Identification ◽  
Beer Spoilage

scholarly journals An improved plate culture procedure for the rapid detection of beer-spoilage lactic acid bacteria

2014 ◽  
Vol 120 (2) ◽  
pp. 127-132 ◽  
Author(s):  
Yang Deng ◽  
Junyan Liu ◽  
Huiping Li ◽  
Lin Li ◽  
Jingxia Tu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rapid Detection ◽  
Beer Spoilage ◽  
Culture Procedure

Effects of morphological changes in beer-spoilage lactic acid bacteria on membrane filtration in breweries

2007 ◽  
Vol 104 (4) ◽  
pp. 334-338 ◽  
Author(s):  
Shizuka Asano ◽  
Koji Suzuki ◽  
Kazumaru Iijima ◽  
Yasuo Motoyama ◽  
Hidetoshi Kuriyama ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Membrane Filtration ◽  
Morphological Changes ◽  
Beer Spoilage

Dipstick Assay for Rapid Detection of Beer Spoilage Organisms

2018 ◽  
Vol 101 (6) ◽  
pp. 1913-1919 ◽  
Author(s):  
Harish K Janagama ◽  
Tam Mai ◽  
Sukkhyun Han ◽  
Lourdes M Nadala ◽  
Cesar Nadala ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rapid Detection ◽  
Cost Effective ◽  
Lactobacillus Brevis ◽  
Test System ◽  
Wild Yeast ◽  
Brewing Process ◽  
Beer Spoilage ◽  
Enrichment Methods

Abstract Background: Beer spoilage caused by wild yeast and bacteria is a major concern to both commercial and home brewers. Objective: To address this problem, Molecular Epidemiology Inc. (MEI, Seattle, WA) has developed a beer spoilage organism detection kit consisting of an enrichment media (BSE) and a multiplex PCR DNA dipstick that simultaneously detects these organisms within 2 h following enrichment. Methods: The kit was tested by using samples obtained from breweries located in the Greater Seattle area. Samples were spiked with the target microbes, when necessary, and used for assessing the performance characteristics of the DNA dipstick assay. Microbial enumerations were performed as per the standard microbiological plating methods. The suitability of the BSE medium to support the growth of beer spoilage microbes was compared with the industry-approved NBB-C medium (Dohler, Darmstadt, Germany). Results: Inclusivity (a panel of 50 isolates) and Exclusivity (a panel of 92 isolates) testing indicated that the dipstick assay can exclusively detect the indicated target beer spoilage microbes. When compared with the NBB-C medium (Dohler, Darmstadt, Germany) approved by the European Brewers Convention for beer spoilage organisms, the BSE medium supported faster growth of critical spoilage lactic acid bacteria such as Lactobacillus brevis, L. lindneri, and Pediococcus damnosus. Conclusions: The beer spoilage organism detection kit has a detection limit of 10 cells/mL. Highlights: The kit can be used at different stages of the brewing process, thus offering a convenient, cost effective, and faster test system for brewers interested in monitoring the quality of their product.

A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria

Food Control ◽  
2010 ◽  
Vol 21 (4) ◽  
pp. 426-430 ◽  
Author(s):  
Huaxi Yi ◽  
Lanwei Zhang ◽  
Yanfeng Tuo ◽  
Xue Han ◽  
Ming Du
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rapid Detection ◽  
Novel Method

scholarly journals Development of detection medium for hard-to-culture beer-spoilage lactic acid bacteria

2008 ◽  
Vol 104 (5) ◽  
pp. 1458-1470 ◽  
Author(s):  
K. Suzuki ◽  
S. Asano ◽  
K. Iijima ◽  
H. Kuriyama ◽  
Y. Kitagawa
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Beer Spoilage ◽  
Detection Medium
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