Identification of differential anti-neoplastic activity of copper bis(thiosemicarbazones) that is mediated by intracellular reactive oxygen species generation and lysosomal membrane permeabilization

2015 ◽  
Vol 152 ◽  
pp. 20-37 ◽  
Author(s):  
Christian Stefani ◽  
Zaynab Al-Eisawi ◽  
Patric J. Jansson ◽  
Danuta S. Kalinowski ◽  
Des R. Richardson
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Oxygen Species ◽  
Lysosomal Membrane Permeabilization

scholarly journals Titanium dioxide induces apoptosis under UVA irradiation via the generation of lysosomal membrane permeabilization-dependent reactive oxygen species in HaCaT cells

2021 ◽  
Author(s):  
In Young Kim ◽  
Tae Geol Lee ◽  
Vytas Reipa ◽  
Min Beom Heo
Keyword(s):  
Reactive Oxygen Species ◽  
Titanium Dioxide ◽  
Reactive Oxygen ◽  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Ros Generation ◽  
Hacat Cells ◽  
Oxygen Species ◽  
Lysosomal Membrane Permeabilization ◽  
Uva Irradiation

Abstract Background Titanium dioxide nanoparticles (TiO2 NPs) are important nanomaterials with wide commercial applications. While the small size of TiO2 NPs is useful in various applications, their biosafety should be evaluated further. In this study, we investigated the cytotoxicity of TiO2 NPs in the presence and absence of UVA irradiation in human keratinocyte HaCaT cells.Results TiO2 NPs did not significantly affect cell viability in the absence of UVA irradiation. However, UVA-irradiated TiO2 NPs induced caspase-dependent apoptosis. Exposure of HaCaT cells to TiO2 NPs and UVA resulted in reactive oxygen species (ROS) generation, and lysosomal membrane permeabilization (LMP); both effects were absent without UVA irradiation. An analysis of the relationship between LMP and ROS, using CA-074 as a cathepsin inhibitor or NAC as an antioxidant, showed that LMP stimulates ROS generation under these conditions.Conclusion These results imply that LMP-dependent oxidative stress plays a critical role in the UVA phototoxicity of TiO2 NPs in HaCaT cells.

Lipopolysaccharide treatment reduces rat platelet aggregation independent of intracellular reactive-oxygen species generation

Platelets ◽  
2011 ◽  
Vol 23 (3) ◽  
pp. 195-201 ◽  
Author(s):  
M. Elisa Lopes-Pires ◽  
André L. Casarin ◽  
Fernanda G. Pereira-Cunha ◽  
Irene Lorand-Metze ◽  
Edson Antunes ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Platelet Aggregation ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species

scholarly journals Role of p85α in neutrophil extra- and intracellular reactive oxygen species generation

Oncotarget ◽  
2016 ◽  
Vol 7 (17) ◽  
pp. 23096-23105 ◽  
Author(s):  
Xing Jun Li ◽  
Lisa Deng ◽  
Stephanie L. Brandt ◽  
Charles B. Goodwin ◽  
Peilin Ma ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species
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