Heterologous expression and characterization of a sigma glutathione S-transferase involved in carbaryl detoxification from oriental migratory locust, Locusta migratoria manilensis (Meyen)

2012 ◽  
Vol 58 (2) ◽  
pp. 220-227 ◽  
Author(s):  
Guohua Qin ◽  
Miao Jia ◽  
Ting Liu ◽  
Xueyao Zhang ◽  
Yaping Guo ◽  
...  
Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 178
Author(s):  
Beibei Li ◽  
Hongmei Li ◽  
Ye Tian ◽  
Nazir Ahmed Abro ◽  
Xiangqun Nong ◽  
...  

Serine protease inhibitors (Serpins) are a broadly distributed superfamily of proteins that exist in organisms with the role of immune responses. Lmserpin1 gene was cloned firstly from Locusta migratoria manilensis and then was detected in all tested stages from eggs to adults and six different tissues through qRT-PCR analysis. The expression was significantly higher in the 3rd instars and within integument. After RNAi treatment, the expression of Lmserpin1 was significantly down-regulated at four different time points. Moreover, it dropped significantly in the fat body and hemolymph at 24 h after treatment. The bioassay results indicated that the mortality of L. migratoria manilensis treated with dsSerpin1 + Metarhizium was significantly higher than the other three treatments. Furthermore, the immune-related genes (PPAE, PPO, and defensin) treated by dsSerpin1 + Metarhizium were significantly down-regulated compared with the Metarhizium treatment, but the activities of phenoloxidase (PO), peroxidase (POD), superoxide dismutase (SOD), glutathione S-transferase (GST), and multifunctional oxidase (MFO) were fluctuating. Our results suggest that Lmserpin1 plays a crucial role in the innate immunity of L. migratoria manilensis. Lmserpin1 probably took part in regulation of melanization and promoted the synthesis of antimicrobial peptides (AMPs).


2014 ◽  
Vol 109 ◽  
pp. 1-5 ◽  
Author(s):  
Guohua Qin ◽  
Ting Liu ◽  
Yaping Guo ◽  
Xueyao Zhang ◽  
Enbo Ma ◽  
...  

2005 ◽  
Vol 5 (1) ◽  
pp. 24-31
Author(s):  
Hamim Sudarsono ◽  
Rosma Hasibuan ◽  
Damayanti Buchori

Biology and Transformation of the Migratory Locust Locusta migratoria manilensis Meyen (Orthoptera: Acrididae) at Several Laboratory Population Density Levels.  The migratory locust, Locusta migratoria manilensis Meyen (Orthoptera: Acrididae) is one of insect pests in Indonesia that undergoes a unique polymorphic biology.  The locust develops a transformation phase which is triggered mainly by high population density.  Its recent outbreaks in several regions of Indonesia cause major concern and control strategies must be developed to strengthen control efforts in the future. Its unique polymorphic phenomenon, therefore, must be investigated thoroughly for the basis of management of the outbreak.  Due to its agility and its long range flying ability, the most realistic technique to study behavioral and morphological change of the locust is in controlled condition at a laboratory or a green house facility. The experiment was aimed to study effects of colony densities on the biology and transformation process of the migratory locust.  More specifically, the experiment was to study effects of population density on the feeding activity, mortality rate, and the ratio of F/C and E/F of the migratory locust L. migratoria manilensis.  The population densities were simulated in colonies at 2, 5, 10, 20, dan 30 couples per cage (cage size: 45x45x90 cm) reared in a green house. Results of the experiment show that, in general, higher densities of the migratory locust colonies tended to feed more rigorously than those of the less dense colonies.  The locust remained in solitary phase at the density of 2-5 couples per cage whereas the transient phase occured at the density of 10-20 couples per cage.  The colonies transformed to gregarious stage when their densities were 30 couples per cage or higher.  Mortality percentage tended to be higher at colonies with higher density, especially for the third instar of the migratory locust. 


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