Effects of temperature, soil moisture and photoperiod on diapause termination and post-diapause development of the wheat blossom midge, Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae)

The wheat blossom midge Sitodiplosis mosellana, one of the most disastrous wheat pests, depends highly on olfactory cues to track suitable plants. To better understand the olfactory recognition mechanisms involved in host selection, in the present study we cloned two S. mosellana adult antenna-specific odorant binding protein (OBP) genes, SmosOBP12 and SmosOBP17, and evaluated bacterially expressed recombinant proteins for their selectivity and sensitivity for host wheat volatiles using the fluorescence-based ligand binding assay. The results showed that both SmosOBPs effectively bound alcohol, ester, ketone, and terpenoid compounds. Particularly, SmosOBP12 had significantly higher affinities (Ki < 10.5 μM) than SmosOBP17 (Ki2 > 0.1 μM) to 3-hexanol, 1-octen-3-ol, D-panthenol, 3-carene, (Z)-3-hexenylacetate, hexyl acetate, methyl salicylate, heptyl acetate, and ethyl heptanoate. Consistently, S. mosellana females were attracted to all these chemicals in a behavioral assay using Y-tube olfactometer. SmosOBP12 also bound aldehyde, but neither bound alkanes. Notably, SmosOBP12 exhibited strong affinity to ocimene (Ki = 8.2 μM) that repelled S. mosellana. SmosOBP17, however, was insensitive to this compound. Taken together, our results indicate that SmosOBP12 may play a greater role than SmosOBP17 in perceiving these biologically active plant volatiles.

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Observations on Rearing and Diapause Termination of Sitodiplosis mosellana (Diptera: Cecidomyiidae) in the Laboratory

Journal of Economic Entomology ◽

10.1093/jee/81.6.1816 ◽

1988 ◽

Vol 81 (6) ◽

pp. 1816-1818 ◽

Cited By ~ 13

Author(s):

C. F. Hinks ◽

J. F. Doane

Keyword(s):

Sitodiplosis Mosellana ◽

Diapause Termination

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AAC Foray red spring wheat

Canadian Journal of Plant Science ◽

10.4141/cjps-2015-007 ◽

2015 ◽

Vol 95 (4) ◽

pp. 799-803 ◽

Cited By ~ 1

Author(s):

P. D. Brown ◽

H. S. Randhawa ◽

J. Mitchell Fetch ◽

S. L. Fox ◽

D. G. Humphreys ◽

...

Keyword(s):

Triticum Aestivum ◽

Spring Wheat ◽

Triticum Aestivum L ◽

Agronomic Performance ◽

Head Blight ◽

Hard Red Spring Wheat ◽

Sitodiplosis Mosellana ◽

Wheat Market ◽

Market Class ◽

Wheat Blossom Midge

Brown, P. D., Randhawa, H. S., Mitchell Fetch, J., Fox, S. L., Humphreys, D. G., Meiklejohn, M., Green, D., Wise, I., Fetch, T., Gilbert, J., McCallum, B. and Menzies, J. 2015. AAC Foray red spring wheat. Can. J. Plant Sci. 95: 799–803. AAC Foray, an orange wheat blossom midge (Sitodiplosis mosellana Géhin) tolerant hard red spring wheat (Triticum aestivum L.), combined high grain yield and good agronomic performance with excellent resistance to leaf and stem rust, and improved resistance to Fusarium head blight. AAC Foray had maturity, straw strength, and test weight similar to the check cultivars. AAC Foray is eligible for grade of the Canada Prairie Spring Red wheat market class.

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Propanil Plus Methyl Parathion on Rice (Oryza sativa)

Weed Science ◽

10.1017/s004317450007497x ◽

1988 ◽

Vol 36 (3) ◽

pp. 335-339 ◽

Cited By ~ 3

Author(s):

Gene D. Wills ◽

Joe E. Street

Keyword(s):

Oryza Sativa ◽

Soil Moisture ◽

Environmental Conditions ◽

Methyl Parathion ◽

Field Experiments ◽

Controlled Environment ◽

Planting Dates ◽

Effects Of Temperature ◽

Effect On Yield ◽

Wilting Point

Effects of propanil [N-(3,4-dichlorophenyl)propanamide] applied to three- to four-leaf rice (Oryza sativaL.) 1 or 7 days before, after, or tank mixed with methyl parathion (O,O-dimethyl-O-4-nitrophenyl phosphorothioate) were determined under different environmental conditions. Field experiments determined the effect on yield of drill-seeded rice, ‘Labelle’ for two planting dates in 1982 and ‘Lemont’ for one planting date in 1986. Treatments were applied at sunrise and at noon. Growth chamber and greenhouse experiments determined the effects of temperature, relative humidity (RH), and soil moisture on response of Labelle rice. In all experiments, propanil, both alone and with methyl parathion, resulted in 20 to 30% leaf burn during the first week after treatment with rapid recovery to less than 10% injury after 3 to 4 weeks. In field experiments, yields were not reduced in the treated rice below that in the untreated controls. In controlled-environment experiments, rice was not injured by propanil plus methyl parathion more than by propanil alone after 2 to 4 weeks. Both treated and untreated rice were injured more by the environmental conditions of high (40 C) temperature, low (40%) RH, and low (near the wilting point) soil moisture than by low (30 C) temperature, high (100%) RH, and flooded soil.

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