Fabrication, mechanical property and in vitro evaluation of poly (L-lactic acid-co-ε-caprolactone) core-shell nanofiber scaffold for tissue engineering

2019 ◽  
Vol 98 ◽  
pp. 48-57 ◽  
Author(s):  
Tingxiao Li ◽  
Lingling Tian ◽  
Susan Liao ◽  
Xin Ding ◽  
Scott A. Irvine ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Tissue Engineering ◽  
Lactic Acid ◽  
Core Shell ◽  
In Vitro Evaluation ◽  
Nanofiber Scaffold

Core–Shell Poly(l-lactic acid)-Hyaluronic Acid Nanofibers for Cell Culture and Pelvic Ligament Tissue Engineering

2021 ◽  
Vol 17 (3) ◽  
pp. 399-406
Author(s):  
Di Zhang ◽  
Xiaobo Huang ◽  
Huaiming Wang ◽  
Yingqi Wei ◽  
Zifeng Yang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Hyaluronic Acid ◽  
Cellular Response ◽  
Pelvic Organ ◽  
Core Shell ◽  
Mouse Bone Marrow ◽  
Gene Markers ◽  
Ligament Tissue Engineering

Pelvic organ prolapse (POP) has become one of the most common serious diseases affecting parous women. Weakening of pelvic ligaments plays an essential role in the pathophysiology of POP. Currently, synthetic materials are widely applied for pelvic reconstructive surgery. However, synthetic nondegradable meshes for POP therapy cannot meet the clinical requirements due to its poor biocompatibility. Herein, we fabricated electrospun core–shell nanofibers of poly(l-lactic acid)-hyaluronic acid (PLLA/HA). After that, we combined them with mouse bone marrow-derived mesenchymal stem cells (mBMSCs) to assess the cellular response and pelvic ligament tissue engineering in vitro. The cellular responses on the composite nanofibers showed that the core–shell structure nanofibers displayed with excellent biocompatibility and enhanced cellular activity without cytotoxicity. Moreover, compared with PLLA nanofibers seeded with mBMSCs, PLLA/HA nanofibers exhibited more cellular function, as revealed by the quantitative real-time polymerase chain reaction (RT-qPCR) for pelvic ligament-related gene markers including Col1a1, Col1a3 and Tnc. These features suggested that this novel core–shell nanofiber is promising in stem cell-based tissue engineering for pelvic reconstruction.

Biocompatabiltiy of Chitosan/Poly(L-Lactic Acid) Composite Scaffolds with Three-Dimensional Honeycomb Patterned Structure

2011 ◽  
Vol 140 ◽  
pp. 29-33
Author(s):  
Ming Yan Zhao ◽  
Li Hua Li ◽  
Guo Dong Sun ◽  
Chang Ren Zhou
Keyword(s):  
Mechanical Property ◽  
Tissue Engineering ◽  
Lactic Acid ◽  
Three Dimensional ◽  
Tissue Engineering Scaffold ◽  
Good Mechanical Property ◽  
Composite Scaffolds ◽  
Ideal Candidate

Three dimensional (3D) scaffolds provide the necessary support for cells to attach, proliferate and differentiate, and define the overall shape of the tissue engineered transplant. In this study, 3D honeycomb patterned chitosan/poly (L-lactic acid) composite scaffolds fabricated by an easy manipulated technique with good mechanical property and cytocompatability, as demonstrated by a previous study. Here we investigated further the in vitro cytocompatibility and spine regeneration in vivo by implanting the construct into male white rabbits for 4 and 8weeks. Results showed that such a honeycomb patterned scaffolds have a good cytocompatibilty. Also, the rabbit spinal defect was perfectly restored. These findings supported that such a 3D honeycomb patterned scaffold is an ideal candidate for the tissue engineering scaffold.

scholarly journals In vitro evaluation of bi-layer silk fibroin scaffolds for gastrointestinal tissue engineering

2014 ◽  
Vol 5 ◽  
pp. 204173141455684 ◽  
Author(s):  
Debra Franck ◽  
Yeun Goo Chung ◽  
Jeannine Coburn ◽  
David L Kaplan ◽  
Carlos R Estrada ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
In Vitro Evaluation ◽  
Gastrointestinal Tissue
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