scholarly journals Solid state nuclear magnetic resonance with magic-angle spinning and dynamic nuclear polarization below 25K

2013 ◽  
Vol 226 ◽  
pp. 100-106 ◽  
Author(s):  
Kent R. Thurber ◽  
Alexey Potapov ◽  
Wai-Ming Yau ◽  
Robert Tycko
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Solid State ◽  
Magnetic Resonance ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Angle Spinning ◽  
Nuclear Magnetic

scholarly journals Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution

2021 ◽  
Vol 2 (1) ◽  
pp. 239-249
Author(s):  
Ivan V. Sergeyev ◽  
Caitlin M. Quinn ◽  
Jochem Struppe ◽  
Angela M. Gronenborn ◽  
Tatyana Polenova
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Large Signal ◽  
Experimental Conditions ◽  
Hiv 1 ◽  
Nuclear Magnetic

Abstract. Dynamic nuclear polarization (DNP)-enhanced magic angle spinning (MAS) nuclear magnetic resonance (NMR) of biological systems is a rapidly growing field. Large signal enhancements make the technique particularly attractive for signal-limited cases, such as studies of complex biological assemblies or at natural isotopic abundance. However, spectral resolution is considerably reduced compared to ambient-temperature non-DNP spectra. Herein, we report a systematic investigation into sensitivity and resolution of 1D and 2D 13C-detected DNP MAS NMR experiments on HIV-1 CA capsid protein tubular assemblies. We show that the magnitude and sign of signal enhancement as well as the homogeneous line width are strongly dependent on the biradical concentration, the dominant polarization transfer pathway, and the enhancement buildup time. Our findings provide guidance for optimal choice of sample preparation and experimental conditions in DNP experiments.

Sign in / Sign up

Export Citation Format

Share Document