Rapid extraction and determination of amphetamines in human urine samples using dispersive liquid–liquid microextraction and solidification of floating organic drop followed by high performance liquid chromatography

2014 ◽  
Vol 94 ◽  
pp. 145-151 ◽  
Author(s):  
Toraj Ahmadi-Jouibari ◽  
Nazir Fattahi ◽  
Mojtaba Shamsipur
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Urine ◽  
High Performance ◽  
Urine Samples ◽  
Human Urine Samples ◽  
Rapid Extraction ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

scholarly journals Analysis of chlorpheniramine in human urine samples using dispersive liquid-liquid microextraction combined with high-performance liquid chromatography

2014 ◽  
Vol 50 (3) ◽  
pp. 551-557 ◽  
Author(s):  
Mehdi Maham ◽  
Vahid Kiarostami ◽  
Syed Waqif-Husain ◽  
Malihe Khoeini Sharifabadi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Urine ◽  
High Performance ◽  
Low Cost ◽  
Test Tube ◽  
Urine Samples ◽  
Human Urine Samples ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

A simple and environmentally friendly microextraction technique was used for determination of chlorpheniramine (CPM), an antihistamine drug, in human urine samples using dispersive liquid-liquid microextraction (DLLME) followed by high performance liquid chromatography with diode array detection (HPLC-DAD). In this extraction technique, an appropriate mixture of acetonitrile (disperser solvent) and carbon tetrachloride (extraction solvent) was rapidly injected into the urine sample containing the target analyte. Tiny droplets of extractant were formed and dispersed into the sample solution and then sedimented at the bottom of the conical test tube by centrifugation. Under optimal conditions, the calibration curve was linear in the range of 0.055-5.5 µg mL-1, with a detection limit of 16.5 ng mL-1. This proposed method was successfully applied to the analysis of real urine samples. Low consumption of toxic organic solvents, simplicity of operation, low cost and acceptable figures of merit are the main advantages of the proposed technique.

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