Online coupling of analytical hydrophobic interaction chromatography with native mass spectrometry for the characterization of monoclonal antibodies and related products

Native Mass Spectrometry

Hyphenation of strong cation exchange chromatography to native mass spectrometry for high throughput online characterization of charge heterogeneity of therapeutic monoclonal antibodies

mAbs ◽

10.1080/19420862.2020.1763762 ◽

2020 ◽

Vol 12 (1) ◽

pp. 1763762

Author(s):

Fengfei Ma ◽

Fahimeh Raoufi ◽

Marc Andre Bailly ◽

Laurence Fayadat-Dilman ◽

Daniela Tomazela

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibodies ◽

Exchange Chromatography ◽

Cation Exchange Chromatography ◽

Charge Heterogeneity ◽

Strong Cation Exchange ◽

Strong Cation Exchange Chromatography ◽

Therapeutic Monoclonal Antibodies

Hydrophobic Interaction Chromatography

Native Hydrophobic Interaction Chromatography Hyphenated to Mass Spectrometry for Characterization of Monoclonal Antibody Minor Variants

Analytical Chemistry ◽

10.1021/acs.analchem.9b04467 ◽

2019 ◽

Vol 91 (24) ◽

pp. 15360-15364 ◽

Cited By ~ 10

Author(s):

Bingchuan Wei ◽

Guanghui Han ◽

Jia Tang ◽

Wendy Sandoval ◽

Yonghua Taylor Zhang

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibody ◽

Hydrophobic Interaction ◽

Separation and Characterization of an IgG2 Antibody Containing a Cyclic Imide in CDR1 of Light Chain by Hydrophobic Interaction Chromatography and Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac702245c ◽

2008 ◽

Vol 80 (9) ◽

pp. 3168-3174 ◽

Cited By ~ 39

Author(s):

John Valliere-Douglass ◽

Laura Jones ◽

Diana Shpektor ◽

Paul Kodama ◽

Alison Wallace ◽

...

Keyword(s):

Mass Spectrometry ◽

Light Chain ◽

Hydrophobic Interaction ◽

Cyclic Imide ◽

Igg2 Antibody

Faculty Opinions recommendation of Online Hydrophobic Interaction Chromatography-Mass Spectrometry for the Analysis of Intact Monoclonal Antibodies.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733321996.793557931 ◽

2019 ◽

Author(s):

John Yates

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibodies ◽

Hydrophobic Interaction ◽

Chromatography Mass Spectrometry

Native Mass Spectrometry, Ion Mobility, and Collision-Induced Unfolding for Conformational Characterization of IgG4 Monoclonal Antibodies

Analytical Chemistry ◽

10.1021/acs.analchem.8b00912 ◽

2018 ◽

Vol 90 (15) ◽

pp. 8865-8872 ◽

Cited By ~ 16

Author(s):

Oscar Hernandez-Alba ◽

Elsa Wagner-Rousset ◽

Alain Beck ◽

Sarah Cianférani

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibodies ◽

Ion Mobility ◽

Collision Induced Unfolding

Hydrophobic Interaction Chromatography

An alternative assay to hydrophobic interaction chromatography for high-throughput characterization of monoclonal antibodies

mAbs ◽

10.1080/19420862.2015.1016694 ◽

2015 ◽

Vol 7 (3) ◽

pp. 553-561 ◽

Cited By ~ 27

Author(s):

Patricia Estep ◽

Isabelle Caffry ◽

Yao Yu ◽

Tingwan Sun ◽

Yuan Cao ◽

...

Keyword(s):

Monoclonal Antibodies ◽

High Throughput ◽

Hydrophobic Interaction ◽

Hydrophobic Interaction Chromatography

Hydrophobic interaction chromatography for the characterization of monoclonal antibodies and related products

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2016.04.004 ◽

2016 ◽

Vol 130 ◽

pp. 3-18 ◽

Cited By ~ 56

Author(s):

Szabolcs Fekete ◽

Jean-Luc Veuthey ◽

Alain Beck ◽

Davy Guillarme

Keyword(s):

Monoclonal Antibodies ◽

Hydrophobic Interaction ◽

Towards a simple on-line coupling of ion exchange chromatography and native mass spectrometry for the detailed characterization of monoclonal antibodies

Journal of Chromatography A ◽

10.1016/j.chroma.2021.462499 ◽

2021 ◽

Vol 1655 ◽

pp. 462499

Author(s):

Amarande Murisier ◽

Bastiaan L. Duivelshof ◽

Szabolcs Fekete ◽

Julien Bourquin ◽

Andrew Schmudlach ◽

...

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibodies ◽

Ion Exchange ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Detailed Characterization ◽

On Line ◽

Line Coupling

Online Hydrophobic Interaction Chromatography–Mass Spectrometry for the Analysis of Intact Monoclonal Antibodies

Analytical Chemistry ◽

10.1021/acs.analchem.8b01865 ◽

2018 ◽

Vol 90 (12) ◽

pp. 7135-7138 ◽

Cited By ~ 27

Author(s):

Bifan Chen ◽

Ziqing Lin ◽

Andrew J. Alpert ◽

Cexiong Fu ◽

Qunying Zhang ◽

...

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibodies ◽

Hydrophobic Interaction ◽

Chromatography Mass Spectrometry

Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry

Analytical Chemistry ◽

10.1021/acs.analchem.8b03773 ◽

2018 ◽

Vol 90 (21) ◽

pp. 13013-13020 ◽

Cited By ~ 20

Author(s):

Yuetian Yan ◽

Anita P. Liu ◽

Shunhai Wang ◽

Thomas J. Daly ◽

Ning Li

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibodies ◽

Exchange Chromatography ◽

Cation Exchange Chromatography ◽

Charge Heterogeneity ◽

Strong Cation Exchange ◽

Strong Cation Exchange Chromatography ◽

Therapeutic Monoclonal Antibodies