Effect of Hemoperfusion Using Polymyxin B-Immobilized Fibers on Non-Shock Rat Sepsis Model

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Kohei Okamoto ◽  
Shiori Kawasaki ◽  
Etsuro Nakarai ◽  
Taku Miyasho
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Takenori Kato ◽  
Takahiro Sugiura ◽  
Ghada A Daoud ◽  
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Tokujiro Uchida ◽  
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Isao Nagaoka ◽  
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Masataka Nagayama ◽  
Takahiro Miki

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Vol 26 (2) ◽  
pp. 187-193 ◽  
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Shin Kato ◽  
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...  

Author(s):  
H.M. Mazzone ◽  
G. Wray ◽  
R. Zerillo

The fungal pathogen of the Dutch elm disease (DED), Ceratocystis ulmi (Buisman) C. Moreau, has eluded effective control since its introduction in the United States more than sixty years ago. Our studies on DED include establishing biological control agents against C. ulmi. In this report we describe the inhibitory action of the antibiotic polymyxin B on the causal agent of DED.In screening a number of antibiotics against C. ulmi, we observed that filter paper discs containing 300 units (U) of polymyxin B (Difco Laboratories) per disc, produced zones of inhibition to the fungus grown on potato dextrose agar or Sabouraud agar plates (100mm x 15mm), Fig. 1a. Total inhibition of fungal growth on a plate occurred when agar overlays containing fungus and antibiotic (polymyxin B sulfate, ICN Pharmaceuticals, Inc.) were poured on the underlying agar growth medium. The agar overlays consisted of the following: 4.5 ml of 0.7% agar, 0.5 ml of fungus (control plate); 4.0 ml of 0.7% agar, 0.5 ml of fungus, 0.5 ml of polymyxin B sulfate (77,700 U). Fig. 1, b and c, compares a control plate and polymyxin plate after seven days.


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