This chapter reviews regional accessibility and relationship between regional accessibility, the logistic infrastructure and regional economic development. The purpose of this chapter is to emphasize the complexity and causality of this relationship. Transport infrastructure is an important policy instrument to promote regional economic development. In addition, development of logistics is a very important part of the transport policy, while accessibility is an important determinant of the attractiveness of regions for logistics activities. Accessibility indicators measure the benefits households and firms in a region enjoy from the existence and use of the transport infrastructure. Economic development may determine transportation needs and lead to infrastructure improvements and accessibility. The theoretically is defined and empirical evidence that transport accessibility suggests that there is a link between the accessibility of the region and its competitiveness and, therefore, regional economic growth.