EW-7197, an ALK5 inhibitor, prevents granulation tissue formation after bare metallic stent placement in a rat urethral model

Abstract Minimally invasive therapies using stent technology are currently limited by stent-induced granulation tissue formation adjacent to the stent. The effectiveness of photothermal therapy (PTT) using a gold nanoparticle (AuNP)-coated stent for treating stent-induced granulation tissue formation in the rat esophagus was investigated. All experiments were approved by the animal research committee of our institution. An AuNP-coated, self-expandable metallic stent (SEMS) was produced to conduct PTT under near-infrared laser irradiation. Forty rats were randomly divided into four groups (10 rats each). The animals in group A (non-coated SEMS) and group B (AuNP-coated SEMS with local heating at 65°C at 4 weeks) were sacrificed 4 weeks after stent placement. The rats in group C (AuNP-coated SEMS with local heating at 65°C at 4 weeks) and group D (AuNP-coated SEMS with local heating at 65°C at 4 and 8 weeks) were sacrificed 8 weeks after stent placement. The effectiveness of local heating was assessed by histopathology. All procedures were successful in all of the animals. Seven rats were excluded because of stent migration (n = 2) and death (n = 5). Granulation tissue formation-related variables were significantly higher in group A than in groups B-D (all p < 0.05). HSP70 and TUNEL expression were significantly lower in group A than in groups B-D (all p < 0.05). Granulation tissue formation-related variables were significantly higher in group C than in groups B and D (all p < 0.05). PTT using AuNP-coated SEMS successfully treated granulation tissue formation after stent placement in the rat esophagus.

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Evaluation of Formation of Granulation Tissue Caused by Metallic Stent Placement in a Rat Urethral Model

Journal of Vascular and Interventional Radiology ◽

10.1016/j.jvir.2010.07.026 ◽

2010 ◽

Vol 21 (12) ◽

pp. 1884-1890 ◽

Cited By ~ 5

Author(s):

Yong-Dong Li ◽

Ho-Young Song ◽

Jin Hyoung Kim ◽

Cheol-Woong Woo ◽

Jung-Hoon Park ◽

...

Keyword(s):

Granulation Tissue ◽

Stent Placement ◽

Metallic Stent ◽

Metallic Stent Placement ◽

Urethral Model

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Abstract No. 571 EW-7197–eluting nano-fiber covered self-expandable metallic stent to prevent granulation tissue formation in a canine urethral model

Journal of Vascular and Interventional Radiology ◽

10.1016/j.jvir.2018.01.616 ◽

2018 ◽

Vol 29 (4) ◽

pp. S238-S239

Author(s):

J. Park ◽

K. Han ◽

D. Lee ◽

M. Kim ◽

K. Kim ◽

...

Keyword(s):

Granulation Tissue ◽

Metallic Stent ◽

Tissue Formation ◽

Granulation Tissue Formation ◽

Expandable Metallic Stent ◽

Nano Fiber ◽

Urethral Model

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Photothermal therapy via a gold nanoparticle-coated stent for treating stent-induced granulation tissue formation in the rat esophagus

Scientific Reports ◽

10.1038/s41598-021-90182-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Young Chul Cho ◽

Jeon Min Kang ◽

Wooram Park ◽

Dong-Hyun Kim ◽

Ji Hoon Shin ◽

...

Keyword(s):

Gold Nanoparticle ◽

Granulation Tissue ◽

Stent Placement ◽

Photothermal Therapy ◽

Near Infrared ◽

Local Heating ◽

Metallic Stent ◽

Tissue Formation ◽

Granulation Tissue Formation ◽

Group A

AbstractMinimally invasive therapies using stent technology are currently limited by stent-induced granulation tissue formation adjacent to the stent. The effectiveness of photothermal therapy (PTT) using a gold nanoparticle (AuNP)-coated stent for treating stent-induced granulation tissue formation in the rat esophagus was investigated. All experiments were approved by the animal research committee of our institution. An AuNP-coated, self-expandable metallic stent (SEMS) was produced to conduct PTT under near-infrared laser irradiation. Forty rats were randomly divided into four groups (10 rats each). The animals in group A (non-coated SEMS) and group B (AuNP-coated SEMS with local heating at 65 °C at 4 weeks) were sacrificed 4 weeks after stent placement. The rats in group C (AuNP-coated SEMS with local heating at 65 °C at 4 weeks) and group D (AuNP-coated SEMS with local heating at 65 °C at 4 and 8 weeks) were sacrificed 8 weeks after stent placement. The effectiveness of local heating was assessed by histopathology. All procedures were successful in all of the animals. Seven rats were excluded because of stent migration (n = 2) and death (n = 5). Granulation tissue formation-related variables were significantly higher in group A than in groups B–D (all p < 0.05). Heat-shock protein 70 (HSP70) and TUNEL expression were significantly lower in group A than in groups B–D (all p < 0.05). Granulation tissue formation-related variables were significantly higher in group C than in groups B and D (all p < 0.05). PTT using AuNP-coated SEMS successfully treated granulation tissue formation after stent placement in the rat esophagus.

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EW-7197 eluting nano-fiber covered self-expandable metallic stent to prevent granulation tissue formation in a canine urethral model

PLoS ONE ◽

10.1371/journal.pone.0192430 ◽

2018 ◽

Vol 13 (2) ◽

pp. e0192430 ◽

Cited By ~ 7

Author(s):

Kichang Han ◽

Jung-Hoon Park ◽

Su-Geun Yang ◽

Deok Hee Lee ◽

Jiaywei Tsauo ◽

...

Keyword(s):

Granulation Tissue ◽

Metallic Stent ◽

Tissue Formation ◽

Granulation Tissue Formation ◽

Expandable Metallic Stent ◽

Nano Fiber ◽

Urethral Model

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Therapeutic effect of local photothermal heating of gold nanoparticle-coated self-expandable metallic stents for suppressing granulation tissue formation in the mouse colon

PLoS ONE ◽

10.1371/journal.pone.0249530 ◽

2021 ◽

Vol 16 (4) ◽

pp. e0249530

Author(s):

Yeong-Cheol Heo ◽

Dong-Kyoon Han ◽

Min Tae Kim

Keyword(s):

Therapeutic Effect ◽

Granulation Tissue ◽

Stent Placement ◽

Local Heating ◽

Tissue Formation ◽

Sems Placement ◽

Granulation Tissue Formation ◽

Mouse Colon ◽

Group A ◽

Group B

Purpose To investigate the therapeutic effect of local photothermal (PT) heating on suppression of stent-induced granulation tissue formation in mouse colon. Materials and methods A gold nanoparticle (GNP)-coated self-expandable metallic stent (SEMS) was prepared using a two-step synthesis process for local PT heating under near-infrared laser irradiation. Twenty-four mice were randomly divided into two groups of 12 and subjected to SEMS placement in the colon. Group A received a GNP-coated SEMS without local heating and Group B received a GNP-coated SEMS and underwent local heating at 55°C after SEMS placement. The therapeutic effect of local heating was assessed by comparing the histopathological, immunohistochemical, and endoscopic results. Results Four mice were excluded because of stent migration (n = 3, group B) or death (n = 1, group A). Stent-induced granulation tissue-related variables were significantly lower in group B than in group A (p < 0.001). In vivo endoscopic images, 4 weeks after stent placement, showed granulation tissue formation over the wire mesh in group A and relatively good patency of the stented colon with no definite irregularities in group B. There was more vascular endothelial growth factor (VEGF) positivity in group A than in group B. Conclusion Local PT heating suppresses granulation tissue formation after stent placement in mouse colon.

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