scholarly journals Therapeutic effect of local photothermal heating of gold nanoparticle-coated self-expandable metallic stents for suppressing granulation tissue formation in the mouse colon

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249530
Author(s):  
Yeong-Cheol Heo ◽  
Dong-Kyoon Han ◽  
Min Tae Kim
Keyword(s):  
Therapeutic Effect ◽  
Granulation Tissue ◽  
Stent Placement ◽  
Local Heating ◽  
Tissue Formation ◽  
Sems Placement ◽  
Granulation Tissue Formation ◽  
Mouse Colon ◽  
Group A ◽  
Group B

Purpose To investigate the therapeutic effect of local photothermal (PT) heating on suppression of stent-induced granulation tissue formation in mouse colon. Materials and methods A gold nanoparticle (GNP)-coated self-expandable metallic stent (SEMS) was prepared using a two-step synthesis process for local PT heating under near-infrared laser irradiation. Twenty-four mice were randomly divided into two groups of 12 and subjected to SEMS placement in the colon. Group A received a GNP-coated SEMS without local heating and Group B received a GNP-coated SEMS and underwent local heating at 55°C after SEMS placement. The therapeutic effect of local heating was assessed by comparing the histopathological, immunohistochemical, and endoscopic results. Results Four mice were excluded because of stent migration (n = 3, group B) or death (n = 1, group A). Stent-induced granulation tissue-related variables were significantly lower in group B than in group A (p < 0.001). In vivo endoscopic images, 4 weeks after stent placement, showed granulation tissue formation over the wire mesh in group A and relatively good patency of the stented colon with no definite irregularities in group B. There was more vascular endothelial growth factor (VEGF) positivity in group A than in group B. Conclusion Local PT heating suppresses granulation tissue formation after stent placement in mouse colon.

scholarly journals Photothermal Therapy via a Gold Nanoparticle-Coated Stent for Treating Stent-Induced Granulation Tissue Formation in the Rat Esophagus

2020 ◽  
Author(s):  
Young Chul Cho ◽  
Jeon Min Kang ◽  
Wooram Park ◽  
Dong-Hyun Kim ◽  
Ji Hoon Shin ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Granulation Tissue ◽  
Stent Placement ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Local Heating ◽  
Metallic Stent ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Group A

Abstract Minimally invasive therapies using stent technology are currently limited by stent-induced granulation tissue formation adjacent to the stent. The effectiveness of photothermal therapy (PTT) using a gold nanoparticle (AuNP)-coated stent for treating stent-induced granulation tissue formation in the rat esophagus was investigated. All experiments were approved by the animal research committee of our institution. An AuNP-coated, self-expandable metallic stent (SEMS) was produced to conduct PTT under near-infrared laser irradiation. Forty rats were randomly divided into four groups (10 rats each). The animals in group A (non-coated SEMS) and group B (AuNP-coated SEMS with local heating at 65°C at 4 weeks) were sacrificed 4 weeks after stent placement. The rats in group C (AuNP-coated SEMS with local heating at 65°C at 4 weeks) and group D (AuNP-coated SEMS with local heating at 65°C at 4 and 8 weeks) were sacrificed 8 weeks after stent placement. The effectiveness of local heating was assessed by histopathology. All procedures were successful in all of the animals. Seven rats were excluded because of stent migration (n = 2) and death (n = 5). Granulation tissue formation-related variables were significantly higher in group A than in groups B-D (all p < 0.05). HSP70 and TUNEL expression were significantly lower in group A than in groups B-D (all p < 0.05). Granulation tissue formation-related variables were significantly higher in group C than in groups B and D (all p < 0.05). PTT using AuNP-coated SEMS successfully treated granulation tissue formation after stent placement in the rat esophagus.

scholarly journals Photothermal therapy via a gold nanoparticle-coated stent for treating stent-induced granulation tissue formation in the rat esophagus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Young Chul Cho ◽  
Jeon Min Kang ◽  
Wooram Park ◽  
Dong-Hyun Kim ◽  
Ji Hoon Shin ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Granulation Tissue ◽  
Stent Placement ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Local Heating ◽  
Metallic Stent ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Group A

AbstractMinimally invasive therapies using stent technology are currently limited by stent-induced granulation tissue formation adjacent to the stent. The effectiveness of photothermal therapy (PTT) using a gold nanoparticle (AuNP)-coated stent for treating stent-induced granulation tissue formation in the rat esophagus was investigated. All experiments were approved by the animal research committee of our institution. An AuNP-coated, self-expandable metallic stent (SEMS) was produced to conduct PTT under near-infrared laser irradiation. Forty rats were randomly divided into four groups (10 rats each). The animals in group A (non-coated SEMS) and group B (AuNP-coated SEMS with local heating at 65 °C at 4 weeks) were sacrificed 4 weeks after stent placement. The rats in group C (AuNP-coated SEMS with local heating at 65 °C at 4 weeks) and group D (AuNP-coated SEMS with local heating at 65 °C at 4 and 8 weeks) were sacrificed 8 weeks after stent placement. The effectiveness of local heating was assessed by histopathology. All procedures were successful in all of the animals. Seven rats were excluded because of stent migration (n = 2) and death (n = 5). Granulation tissue formation-related variables were significantly higher in group A than in groups B–D (all p < 0.05). Heat-shock protein 70 (HSP70) and TUNEL expression were significantly lower in group A than in groups B–D (all p < 0.05). Granulation tissue formation-related variables were significantly higher in group C than in groups B and D (all p < 0.05). PTT using AuNP-coated SEMS successfully treated granulation tissue formation after stent placement in the rat esophagus.

scholarly journals Comparison of vacuum assisted closure therapy with conventional dressing in the management of necrotizing fasciitis wound

2021 ◽  
Vol 8 (12) ◽  
pp. 3587
Author(s):  
Birbal Kumar ◽  
R. S. Mohil ◽  
Sajith K. Mohan ◽  
Navnik Singh Bhardwaj ◽  
Arush Pasricha ◽  
...  
Keyword(s):  
Necrotizing Fasciitis ◽  
Granulation Tissue ◽  
Skin Grafting ◽  
Tissue Formation ◽  
Vacuum Assisted Closure ◽  
Granulation Tissue Formation ◽  
Vac Therapy ◽  
Control Frequency ◽  
Group A ◽  
Group B

Background: The purpose of our study was to compare the effect of vacuum assisted closure (VAC) therapy and conventional dressings in patients with open wounds due to necrotizing fasciitis (NF) on the basis of healing rate, infection control, frequency of dressing and pain score.Methods: The study evaluated 50 patients admitted with NF requiring surgery over a period      of 18 months.  The patients were randomized to two groups. In group A patients, the wounds were managed with conventional dressings and in group B patients, negative pressure wound therapy (NPWT) was applied. Serial assessment of both groups was done for four weeks. The parameters including size of wound, wound bed, granulation tissue formation, color, amount and odor of exudate, edema, frequency of dressing, re-debridement and pain were monitored and analysed.Results: In our study, patients with NF wounds who underwent VAC therapy had earlier granulation tissue formation, resolution of infection and readiness for skin grafting. The frequency of dressing, requirement of re-debridement, resolution of edema, odor, skin maceration, inflammation around wound and pain significantly reduced in group B (VAC) when compared to conventional dressing group.Conclusions: When compared to the conventional dressing on NF wound, application of VAC helped in early appearance of granulation tissue, significant reduction of inflammation, wound odor, exudate, need for re-debridement, frequency of dressing and pain. Thus, VAC dressing can be considered as a better option in the management of NF wounds.

scholarly journals Surgical outcomes in acute dacryocystitis patients undergoing endonasal endoscopic dacryocystorhinostomy with or without silicone tube intubation

2021 ◽  
Vol 14 (6) ◽  
pp. 844-848
Author(s):  
Bo Yu ◽  
◽  
Jia-Ying Sun ◽  
Qian Ye ◽  
Yun-Hai Tu ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Tissue Formation ◽  
Success Rates ◽  
Granulation Tissue Formation ◽  
Treatment Groups ◽  
Group A ◽  
Silicone Intubation ◽  
Silicone Tube Intubation ◽  
Group B

AIM: To establish the necessity of silicone tube intubation in acute dacryocystitis (AD) patients undergoing endonasal endoscopic dacryocystorhinostomy (En-DCR). METHODS: Patients presenting with unilateral AD were randomly assigned to two treatment groups. En-DCR procedures were performed following lacrimal abscess formation, with the operation being performed with silicone intubation for patients in group B but not group A. Functional success was defined by an absence of additional AD episodes, no epiphora, and ostium patency as established via endoscopic evaluation or fluorescein irrigation. Operative success rates and demographic variables were compared between treatment groups. RESULTS: In total, 66 patients were analyzed in the present study (33 per group), with complete postoperative data having been successfully collected from 27 and 22 patients in group A and group B, respectively. All patients exhibited complete resolution of acute inflammation. Upon follow-up, granulation tissue was detected around the ostium at higher rates in group B (9/22, 40.9%) relative to group A (4/27, 14.8%). At the 12-month follow-up time point, patients in group A exhibited higher success rates (25/27, 92.6%) relative to patients in group B (20/22, 90.9%), but this difference was not significant. Cases of lacrimal passage reconstruction failure in both groups were attributed to excessive fibrous and/or granulation tissue formation proximal to the intranasal ostium. CONCLUSION: Given that these two operative approaches are associated with similar rates of operative success and in light of differences in granulation tissue formation, cost, and operative duration, these data do not support the routine silicone intubation of AD patients following En-DCR surgery.

scholarly journals KHASIAT DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP PEMBENTUKAN JARINGAN GRANULASI DAN REEPITELISASI PENYEMBUHAN LUKA TERBUKA KULIT KELINCI

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Suci Ariani
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Temperature Changes ◽  
Traditional Medicines ◽  
Epithelial Regeneration ◽  
Microscopic Features ◽  
Group A ◽  
Group B

Latar Belakang: Luka merupakan keadaan dimana kontinuitas jaringan rusak oleh karena trauma dari benda tajam atau tumpu, perubahan suhu, kimiawi, listrik, radiasi, atau gigitan hewan. Proses penyembuhan luka bisa dipercepat dengan menggunakan pengobatan tradisional, salah satunya adalah tanaman binahong (Anredera cordifolia (Ten.) Steenis). Tujuan: Untuk melihat khasiat daun binahong terhadap pembentukan jaringan granulasi dan reepitelisasi penyembuhan luka terbuka kulit kelinci secara makroskopik dan mikroskopik. Metode: Penelitian ini menggunakan metode eksperimental dengan subjek 5 ekor kelinci. Satu ekor kelinci untuk meilhat struktur jaringan kulit normal kelinci. Empat ekor kelinci dibuat luka, pada punggung kanan diberikan daun binahong dan luka pada punggung kiri tidak diberikan daun binahong. Kelompok A terdiri dari dua ekor kelinci yang diambil dan dilihat jaringan luka pada hari kelima, kelompok B terdiri dari dua ekor kelinci yang diambil dan dilihat jaringan lukanya pada hari ke-14. Hasil: Secara makroskopik luka yang diberi daun binahong terlihat lebih kecil dan kering, sedangkan yang tidak diberi daun binahong terlihat luka masih dalam, dan kemerahan. Secara mikroskopik luka yang diberi daun binahong terbentuk jaringan granulasi yang lebih banyak dan reepitelisasi lebih cepat dibandingkan dengan yang tidak diberi daun binahong. Simpulan: Pemberian daun binahong pada luka membantu penyembuhan luka dengan pembentukan jaringan granulasi yang lebih banyak dan reepitelisasi terjadi lebih cepat dibandingkan dengan luka yang tidak diberi daun binahong.Kata Kunci: Luka terbuka, daun binahong, jaringan granulasi, reepitelisasi.Background: Wound is a condition when the continuity of tissue are damaged by the trauma of sharp or blunt objects, temperature changes, chemicals, electricity, radiation, or animal bites. The process of wound healing can be accelerated by using traditional medicines, one of them is a plant called Binahong (Anredera cordifolia (Ten.) Steenis). Objective: To observe the influences of binahong leaf in granulation tissue formation and epithelial regeneration of an open wound healing of skin rabbit, both macroscopic and microscopic features. Method: Experimental research employed five adult rabbits as the subject: one rabbit as a control – to observe the structure of rabbit skin tissue. The four rabbits are made wounds, to the back right were given binahong leafs and wounds to the back left were not. Group A and B - two rabbits in each group. Rabbits in group A are used to observe the wounded tissue in the fifth day. Rabbits in group B are used to observe the wounded tissue in the fourteenth day. Results: The macroscopic result of wounds with binahong had smaller shape and dry, while the wound without binahong leafs are still look deeper, wet, and redness. By microscopic examination, wounds with binahong leafs formed granulation tissue and epithelial regeneration more and faster than those which not given binahong leafs. Conclusions: Binahong leafs help in healing the wounds with forming more granulation tissue and faster in epithelial regeneration than the wounds without binahong leafs.Keywords: Open wounds, binahong leaf, granulation tissue, epithelial regeneration.

scholarly journals Bioreducible Polymer–delivered siRNA Targeting MMP-9: Suppression of Granulation Tissue Formation after Bare Metallic Stent Placement in a Rat Urethral Model

Radiology ◽  
2014 ◽  
Vol 271 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Jung-Hoon Park ◽  
Jin Hyoung Kim ◽  
Eun-Young Kim ◽  
Jinoo Kim ◽  
Ho-Young Song ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Stent Placement ◽  
Metallic Stent ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Metallic Stent Placement ◽  
Urethral Model

scholarly journals IN-1233, an ALK-5 Inhibitor: Prevention of Granulation Tissue Formation after Bare Metallic Stent Placement in a Rat Urethral Model

Radiology ◽  
2010 ◽  
Vol 255 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Jin Hyoung Kim ◽  
Ho-Young Song ◽  
Jung-Hoon Park ◽  
Hyun-Jung Yoon ◽  
Hyung Gyu Park ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Stent Placement ◽  
Metallic Stent ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Alk 5 ◽  
Metallic Stent Placement ◽  
Urethral Model

scholarly journals Bilateral Choanal Atresia and Endoscopic Surgery: A Chance for CHARGE Patients

2021 ◽  
Vol 10 (13) ◽  
pp. 2951
Author(s):  
Maria Baldovin ◽  
Diego Cazzador ◽  
Claudia Zanotti ◽  
Giuliana Frasson ◽  
Athanasios Saratziotis ◽  
...  
Keyword(s):  
Congenital Malformation ◽  
Endoscopic Surgery ◽  
Granulation Tissue ◽  
Choanal Atresia ◽  
Tissue Formation ◽  
Endoscopic Repair ◽  
Granulation Tissue Formation ◽  
Charge Association ◽  
Cohort Data

Bilateral choanal atresia (CA) is a rare congenital malformation frequently associated with other anomalies. CHARGE association is closely linked to bilateral CA. The aim of this study was to describe the outcomes of the endoscopic repair in bilateral CA, and to assess the role of postoperative nasal stenting in two cohorts of CHARGE-associated and non-syndromic CA. Thirty-nine children were retrospectively analyzed (16 patients had CHARGE-associated CA). The rate of postoperative neochoanal restenosis was 31.3% in the CHARGE population, and 47.8% in the non-syndromic CA cohort. Data on postoperative synechiae and granulation tissue formation, need for endonasal toilette and dilation procedures, and number of procedures per patient were presented. Stent positioning led to a higher number of postoperative dilation procedures per patient in the non-syndromic cohort (p = 0.018), and to a higher rate of restenosis both in the CHARGE-associated, and non-syndromic CA populations. Children with CHARGE-associated and non-syndromic bilateral CA benefitted from endonasal endoscopic CA correction. The postoperative application of an endonasal stent should be carefully evaluated.

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