Multiplex real-time PCR assay for simultaneous quantitation of human cytomegalovirus and herpesvirus-6 in polymorphonuclear and mononuclear cells of transplant recipients

2006 ◽  
Vol 132 (1-2) ◽  
pp. 77-84 ◽  
Author(s):  
Karine Pradeau ◽  
Ludovic Couty ◽  
Jean-Christophe Szelag ◽  
Pascal Turlure ◽  
Florence Rolle ◽  
...  
Keyword(s):  
Real Time ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Mononuclear Cells ◽  
Transplant Recipients ◽  
Pcr Assay ◽  
Herpesvirus 6

scholarly journals Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real-Time PCR Assay

2003 ◽  
Vol 41 (8) ◽  
pp. 3840-3845 ◽  
Author(s):  
C. Mengelle ◽  
K. Sandres-Saune ◽  
C. Pasquier ◽  
L. Rostaing ◽  
J.-M. Mansuy ◽  
...  
Keyword(s):  
Real Time ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Automated Extraction ◽  
Pcr Assay

scholarly journals Multiplex real-time PCR to identify a possible reinfection with different strains of human cytomegalovirus in allogeneic hematopoietic stem cell transplant recipients.

2016 ◽  
Vol 63 (1) ◽  
Author(s):  
Barbara Zawilinska ◽  
Slawa Szostek ◽  
Jolanta Kopec ◽  
Beata Piatkowska-Jakubas ◽  
Magdalena Kosz-Vnenchak
Keyword(s):  
Stem Cell ◽  
Real Time ◽  
Hematopoietic Stem Cell ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Antiviral Treatment ◽  
Cell Transplant ◽  
Transplant Recipients ◽  
Viral Glycoprotein ◽  
Hematopoietic Stem

Human cytomegalovirus (HCMV) infection remains the leading cause of serious contagious complications after allogeneic hematopoietic stem cell transplantation. These infections in HCMV-seropositive recipients can be due to reactivation or reinfection. Different HCMV strains were identified by determining the genotypes isolated from repeatedly tested patients. The UL55 sequences encoding viral glycoprotein B (gB) have been chosen as the target gene. The region, in which the gB precursor protein is cleaved into two fragments by a cellular endoprotease, is characterized by genetic variability, and based on that HCMV is classified into four major genotypes: gB1, gB2, gB3 and gB4. Multiplex real-time PCR assay enabled both, HCMV gB genotyping, as well as simultaneous quantitative assessment of the detected genotypes. This study was carried out in 30 transplant recipients, from whom 105 isolates of HCMV DNA were genotyped. In 40% of recipients, a mixed infection with two or three genotypes was detected. Genotype gB1 dominated in general, and characteristically for mixed infections, the genotype gB3 or gB4 was always present. Although there were no significant differences in the load for each genotype, in case of multiple infections, the number of copies of gB1 genotype was significantly higher when compared to a single gB1 infection. In patients with mixed genotypes, chronic HCMV infections and graft versus host disease were observed more often, as well as antiviral treatment was less effective. It was assumed that these adverse effects can be related to the presence of gB3 and gB4 genotypes.

scholarly journals Quantification of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients by Real-Time PCR

2001 ◽  
Vol 39 (12) ◽  
pp. 4362-4369 ◽  
Author(s):  
F. Griscelli ◽  
M. Barrois ◽  
S. Chauvin ◽  
S. Lastere ◽  
D. Bellet ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Real Time ◽  
Bone Marrow Transplant ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Marrow Transplant ◽  
Transplant Recipients

scholarly journals Detection of Human Cytomegalovirus DNA by Real-Time Quantitative PCR

2000 ◽  
Vol 38 (7) ◽  
pp. 2734-2737 ◽  
Author(s):  
Andreas Nitsche ◽  
Nina Steuer ◽  
Christian Andreas Schmidt ◽  
Olfert Landt ◽  
Heinz Ellerbrok ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Real Time ◽  
Quantitative Pcr ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Blood Donors ◽  
Marrow Transplant ◽  
Pcr Assay ◽  
Real Time Quantitative Pcr ◽  
Transplant Patients

A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.

scholarly journals A Real-Time PCR Assay to Detect Human Cytomegalovirus DNA in Amniotic Fluid of Primarily Infected Women

2008 ◽  
Vol 12 ◽  
pp. e185
Author(s):  
T. Lazzarotto ◽  
B. Guerra ◽  
L. Gabrielli ◽  
F. Baccolini ◽  
M. Lanari ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Real Time ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Pcr Assay
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