Evaluation of a primer-probe energy transfer real-time PCR assay for detection of classical swine fever virus

2010 ◽  
Vol 168 (1-2) ◽  
pp. 259-261 ◽  
Author(s):  
Xing-Juan Zhang ◽  
Hongyan Xia ◽  
Helen Everett ◽  
Olubukola Sosan ◽  
Helen Crooke ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Classical Swine Fever Virus ◽  
Real Time Pcr ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Pcr Assay

Comparison of PCR methods for detection of classical swine fever virus and other pestiviruses

2012 ◽  
Vol 15 (4) ◽  
pp. 615-620
Author(s):  
K. Podgórska ◽  
K. Kamieniecka ◽  
T. Stadejek ◽  
Z. Pejsak
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Diagnostic Methods ◽  
Careful Selection ◽  
Specific Detection ◽  
Selection Of

AbstractClassical swine fever (CSF) is a notifiable, highly contagious disease of swine controlled mainly with costly administrative methods. Swine may be infected not only with classical swine fever virus (CSFV), but also with other, non porcine, genetically and antigenically related pestiviruses. Differentiation of infections with CSFV and other pestiviruses is a crucial element of diagnostics.In the present study two real-time PCR methods and conventional one-tube nested PCR for specific detection of CSFV were compared. Additionally, two methods designed for detection of all pestivirus species real-time SYBR Green I and one-tube nested PCR were included into the study. Analyzed methods varied considerably regarding their sensitivity and specificity, what suggests that careful selection of diagnostic methods and their evaluation on a regular basis is necessary.

Development and application of real-time TaqMan RT-PCR assay for improved detection of classical swine fever virus in slaughtered pigs

2016 ◽  
Vol 50 (6) ◽  
Author(s):  
M. Rout ◽  
G. Saikumar
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Clinical Signs ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Taqman Probe ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Fluorogenic Probe ◽  
Slaughtered Pigs

Classical swine fever (CSF) is an economically devastating disease of pigs. Instrumental to the control of CSF is a well-characterized sensitive assay that can deliver a rapid and accurate diagnosis before the onset of clinical signs. With this objective, a real-time fluorogenic-probe hydrolysis (TaqMan) reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for rapid and specific detection of classical swine fever virus (CSFV) and applied on samples derived from infected slaughtered pigs. A pair of PCR primers targeting 5’ -non-coding region (CSFL1 and CSFR1) in conjunction with a CSFV-specific fluorogenic probe (CSFP1) was designed and assessed in real-time PCR. During PCR, when the target of interest was present, the CSFV specific FAM-labeled TaqMan probe annealed to the amplicon between the forward and reverse primers and was subsequently cleaved via the 5¢-3¢ exonuclease activity of the DNA polymerase resulting in the release of the fluorescent reporter dye. This assay was found to be rapid and strain-specific for CSFV detection.

scholarly journals Development of a reverse transcription multiplex real-time PCR for the detection and genotyping of classical swine fever virus

2009 ◽  
Vol 160 (1-2) ◽  
pp. 111-118 ◽  
Author(s):  
Yu-Liang Huang ◽  
Victor Fei Pang ◽  
Chu-Hsiang Pan ◽  
Tsu-Han Chen ◽  
Ming-Hwa Jong ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Classical Swine Fever Virus ◽  
Real Time Pcr ◽  
Classical Swine Fever ◽  
Fever Virus
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