Development and validation of a novel SYBR Green real-time RT-PCR assay for the detection of classical swine fever virus evaluated on different real-time PCR platforms

2011 ◽  
Vol 174 (1-2) ◽  
pp. 53-59 ◽  
Author(s):  
Lester Josué Pérez ◽  
Heidy Díaz de Arce ◽  
Joan Tarradas ◽  
Rosa Rosell ◽  
Carmen Laura Perera ◽  
...  
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Real Time Pcr ◽  
Classical Swine Fever ◽  
Sybr Green ◽  
Fever Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Development And Validation

Evaluation of a primer-probe energy transfer real-time PCR assay for detection of classical swine fever virus

2010 ◽  
Vol 168 (1-2) ◽  
pp. 259-261 ◽  
Author(s):  
Xing-Juan Zhang ◽  
Hongyan Xia ◽  
Helen Everett ◽  
Olubukola Sosan ◽  
Helen Crooke ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Classical Swine Fever Virus ◽  
Real Time Pcr ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Pcr Assay

Development and application of real-time TaqMan RT-PCR assay for improved detection of classical swine fever virus in slaughtered pigs

2016 ◽  
Vol 50 (6) ◽  
Author(s):  
M. Rout ◽  
G. Saikumar
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Clinical Signs ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Taqman Probe ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Fluorogenic Probe ◽  
Slaughtered Pigs

Classical swine fever (CSF) is an economically devastating disease of pigs. Instrumental to the control of CSF is a well-characterized sensitive assay that can deliver a rapid and accurate diagnosis before the onset of clinical signs. With this objective, a real-time fluorogenic-probe hydrolysis (TaqMan) reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for rapid and specific detection of classical swine fever virus (CSFV) and applied on samples derived from infected slaughtered pigs. A pair of PCR primers targeting 5’ -non-coding region (CSFL1 and CSFR1) in conjunction with a CSFV-specific fluorogenic probe (CSFP1) was designed and assessed in real-time PCR. During PCR, when the target of interest was present, the CSFV specific FAM-labeled TaqMan probe annealed to the amplicon between the forward and reverse primers and was subsequently cleaved via the 5¢-3¢ exonuclease activity of the DNA polymerase resulting in the release of the fluorescent reporter dye. This assay was found to be rapid and strain-specific for CSFV detection.

Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus

2008 ◽  
Vol 147 (1) ◽  
pp. 136-142 ◽  
Author(s):  
M. Le Dimna ◽  
R. Vrancken ◽  
F. Koenen ◽  
S. Bougeard ◽  
A. Mesplède ◽  
...  
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Rt Pcr ◽  
Specific Diagnosis
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