Evaluation of automated nucleic acid extraction methods for virus detection in a multicenter comparative trial

2009 ◽  
Vol 155 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Thomas Bruun Rasmussen ◽  
Åse Uttenthal ◽  
Mikhayil Hakhverdyan ◽  
Sándor Belák ◽  
Philip R. Wakeley ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Virus Detection ◽  
Comparative Trial ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals Evaluation of simple nucleic acid extraction methods for the detection of SARS-CoV-2 in nasopharyngeal and saliva specimens during global shortage of extraction kits

2020 ◽  
Vol 129 ◽  
pp. 104519 ◽  
Author(s):  
Allen Wing-Ho Chu ◽  
Wan-Mui Chan ◽  
Jonathan Daniel Ip ◽  
Cyril Chik-Yan Yip ◽  
Jasper Fuk-Woo Chan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-Time PCR

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Yoonjung Kim ◽  
Mi-Soon Han ◽  
Juwon Kim ◽  
Aerin Kwon ◽  
Kyung-A Lee
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
False Negative ◽  
Virus Detection ◽  
Turnaround Time ◽  
Nasopharyngeal Swab ◽  
Acid Extraction ◽  
Analytical Sensitivity ◽  
Nucleic Acid Extraction ◽  
Negative Results

A total of 84 nasopharyngeal swab specimens were collected from 84 patients. Viral nucleic acid was extracted by three automated extraction systems: QIAcube (Qiagen, Germany), EZ1 Advanced XL (Qiagen), and MICROLAB Nimbus IVD (Hamilton, USA). Fourteen RNA viruses and two DNA viruses were detected using the Anyplex II RV16 Detection kit (Seegene, Republic of Korea). The EZ1 Advanced XL system demonstrated the best analytical sensitivity for all the three viral strains. The nucleic acids extracted by EZ1 Advanced XL showed higher positive rates for virus detection than the others. Meanwhile, the MICROLAB Nimbus IVD system was comprised of fully automated steps from nucleic extraction to PCR setup function that could reduce human errors. For the nucleic acids recovered from nasopharyngeal swab specimens, the QIAcube system showed the fewest false negative results and the best concordance rate, and it may be more suitable for detecting various viruses including RNA and DNA virus strains. Each system showed different sensitivity and specificity for detection of certain viral pathogens and demonstrated different characteristics such as turnaround time and sample capacity. Therefore, these factors should be considered when new nucleic acid extraction systems are introduced to the laboratory.

scholarly journals Comparison of Automated Nucleic Acid Extraction Methods with Manual Extraction

2008 ◽  
Vol 10 (4) ◽  
pp. 311-316 ◽  
Author(s):  
Nicola Dundas ◽  
N. Kristine Leos ◽  
Midori Mitui ◽  
Paula Revell ◽  
Beverly Barton Rogers
Keyword(s):  
Nucleic Acid ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Manual Extraction

scholarly journals Comparison of QIAGEN Automated Nucleic Acid Extraction Methods for CMV Quantitative PCR Testing

2010 ◽  
Vol 133 (4) ◽  
pp. 558-563 ◽  
Author(s):  
Steve Miller ◽  
Henrietta Seet ◽  
Yasmeen Khan ◽  
Carolyn Wright ◽  
Rohan Nadarajah
Keyword(s):  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

Evaluation and optimization of nucleic acid extraction methods for the molecular analysis of bacterial communities associated with corroded carbon steel

Biofouling ◽  
2012 ◽  
Vol 28 (4) ◽  
pp. 363-380 ◽  
Author(s):  
Florence Marty ◽  
Jean-François Ghiglione ◽  
Sandrine Païssé ◽  
Hervé Gueuné ◽  
Laurent Quillet ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Carbon Steel ◽  
Molecular Analysis ◽  
Bacterial Communities ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction
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