scholarly journals Evaluation of simple nucleic acid extraction methods for the detection of SARS-CoV-2 in nasopharyngeal and saliva specimens during global shortage of extraction kits

2020 ◽  
Vol 129 ◽  
pp. 104519 ◽  
Author(s):  
Allen Wing-Ho Chu ◽  
Wan-Mui Chan ◽  
Jonathan Daniel Ip ◽  
Cyril Chik-Yan Yip ◽  
Jasper Fuk-Woo Chan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals Comparison of Automated Nucleic Acid Extraction Methods with Manual Extraction

2008 ◽  
Vol 10 (4) ◽  
pp. 311-316 ◽  
Author(s):  
Nicola Dundas ◽  
N. Kristine Leos ◽  
Midori Mitui ◽  
Paula Revell ◽  
Beverly Barton Rogers
Keyword(s):  
Nucleic Acid ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Manual Extraction

scholarly journals Comparison of QIAGEN Automated Nucleic Acid Extraction Methods for CMV Quantitative PCR Testing

2010 ◽  
Vol 133 (4) ◽  
pp. 558-563 ◽  
Author(s):  
Steve Miller ◽  
Henrietta Seet ◽  
Yasmeen Khan ◽  
Carolyn Wright ◽  
Rohan Nadarajah
Keyword(s):  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

Evaluation and optimization of nucleic acid extraction methods for the molecular analysis of bacterial communities associated with corroded carbon steel

Biofouling ◽  
2012 ◽  
Vol 28 (4) ◽  
pp. 363-380 ◽  
Author(s):  
Florence Marty ◽  
Jean-François Ghiglione ◽  
Sandrine Païssé ◽  
Hervé Gueuné ◽  
Laurent Quillet ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Carbon Steel ◽  
Molecular Analysis ◽  
Bacterial Communities ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

Evaluation of automated nucleic acid extraction methods for virus detection in a multicenter comparative trial

2009 ◽  
Vol 155 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Thomas Bruun Rasmussen ◽  
Åse Uttenthal ◽  
Mikhayil Hakhverdyan ◽  
Sándor Belák ◽  
Philip R. Wakeley ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Virus Detection ◽  
Comparative Trial ◽  
Extraction Methods ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Nasir Ali ◽  
Rita de Cássia Pontello Rampazzo ◽  
Alexandre Dias Tavares Costa ◽  
Marco Aurelio Krieger
Keyword(s):  
Nucleic Acid ◽  
Extraction Method ◽  
Point Of Care ◽  
Extraction Methods ◽  
Vital Role ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Advantages And Disadvantages ◽  
Point Of Care Diagnostics ◽  
Working Principle

Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at thepoint of careorpoint of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.

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