scholarly journals Development of a real-time RT-PCR assay for detection of resistance to oseltamivir in influenza A pandemic (H1N1) 2009 virus using single nucleotide polymorphism probes

2011 ◽  
Vol 173 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Sallene Wong ◽  
Kanti Pabbaraju ◽  
Anita Wong ◽  
Kevin Fonseca ◽  
Steven J. Drews
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Real Time ◽  
Influenza A ◽  
Pandemic H1n1 ◽  
Nucleotide Polymorphism ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Nucleotide ◽  
Pandemic H1n1 2009

Validation of commercial real-time RT-PCR kits for detection of influenza A viruses in porcine samples and differentiation of pandemic (H1N1) 2009 virus in pigs

2011 ◽  
Vol 171 (1) ◽  
pp. 241-247 ◽  
Author(s):  
Françoise Pol ◽  
Stéphane Quéguiner ◽  
Stéphane Gorin ◽  
Céline Deblanc ◽  
Gaëlle Simon
Keyword(s):  
Real Time ◽  
Influenza A ◽  
Pandemic H1n1 ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pandemic H1n1 2009

scholarly journals Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

2021 ◽  
pp. 104063872199481
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A ◽  
Minor Groove Binder ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Infectious Dose ◽  
The Matrix ◽  
Taqman Minor Groove Binder

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

scholarly journals Development of a Diagnostic System for Novel Influenza A(H7N9) Virus Using a Real-Time RT-PCR Assay in Japan

2015 ◽  
Vol 68 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Ikuyo Takayama ◽  
Hitoshi Takahashi ◽  
Mina Nakauchi ◽  
Shiho Nagata ◽  
Masato Tashiro ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
Diagnostic System ◽  
H7n9 Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Novel Influenza A
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