Multiplex real-time PCR and high-resolution melting analysis for detection of white spot syndrome virus, yellow-head virus, and Penaeus monodon densovirus in penaeid shrimp

2011 ◽  
Vol 178 (1-2) ◽  
pp. 16-21 ◽  
Author(s):  
Benjaporn Panichareon ◽  
Paisarn Khawsak ◽  
Warin Deesukon ◽  
Wasana Sukhumsirichart
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
White Spot Syndrome Virus ◽  
High Resolution Melting ◽  
Penaeus Monodon ◽  
Penaeid Shrimp ◽  
White Spot ◽  
Yellow Head Virus ◽  
Melting Analysis

Development of a quantitation approach for total human and male DNA based on real time PCR followed by high resolution melting analysis

2016 ◽  
Vol 37 (21) ◽  
pp. 2734-2741 ◽  
Author(s):  
Santiago Ginart ◽  
Mariela Caputo ◽  
Evguenia Alechine ◽  
Daniel Corach ◽  
Andrea Sala
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
High Resolution Melting ◽  
High Resolution Melting Analysis ◽  
Melting Analysis

Real-time PCR followed by high-resolution melting analysis – a new robust approach to evaluate SCCmectyping of methicillin-resistantStaphylococcus aureus

2019 ◽  
Vol 14 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Yasaman Sadeghi ◽  
Seyed Alireza Salami ◽  
Pegah Kananizadeh ◽  
Sayed-Hamidreza Mozhgani ◽  
Mohammad Reza Pourmand
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
High Resolution Melting ◽  
High Resolution Melting Analysis ◽  
Robust Approach ◽  
Melting Analysis

scholarly journals Real-time PCR High-resolution Melting Analysis for the Species Identification of Meat Products: Focusing on Food Safety and Detection of Meat Adulterations

Thrita ◽  
2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Peyman Gholamnezhad ◽  
Hamed Ahari ◽  
Gholamreza Nikbakht Brujeni ◽  
Seyed Amir Ali Anvar ◽  
Abbas Ali Motalebi
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Species Identification ◽  
Real Time Pcr ◽  
High Resolution Melting ◽  
Meat Products ◽  
Hrm Analysis ◽  
Melting Analysis ◽  
Meat Species ◽  
Meat Samples

Background: Real-time polymerase chain reaction (PCR) and high-resolution melting (HRM) analysis are currently considered as reliable techniques for the species identification of meat-based products and widely used to detect meat adulteration. Objectives: To examine the validity of real-time PCR and HRM analysis to identify meat species in meat-based products. Methods: Meat samples from five species (i.e., cattle, sheep, chicken, turkey, and wild pig) were purchased. Minced meat from the animal species of interest was prepared at the purities of 10%, and 20% and also were prepared as single and mixtures of two species. For molecular assessments, DNA samples were extracted from all the meat samples and subjected to real-time PCR by amplifying a mitochondrial cytochrome b specific for each species. Results: All the meat species studied in this research were successfully detected in the mixed meat samples when separately examined by real-time PCR. High-resolution melting analysis showed that all the meat species of interest were efficiently distinguished when examined simultaneously. Conclusions: The data presented here shows that the real-time PCR and HRM analysis are reliable methods for the identification of meat species used in meat products.

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