Development of single-step multiplex real-time RT-PCR assays for rapid diagnosis of enterovirus 71, coxsackievirus A6, and A16 in patients with hand, foot, and mouth disease

2017 ◽  
Vol 248 ◽  
pp. 92-99 ◽  
Author(s):  
Jiratchaya Puenpa ◽  
Kamol Suwannakarn ◽  
Jira Chansaenroj ◽  
Sompong Vongpunsawad ◽  
Yong Poovorawan
Keyword(s):  
Real Time ◽  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Rapid Diagnosis ◽  
Single Step ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Coxsackievirus A6 ◽  
Foot And Mouth ◽  
Pcr Assays

scholarly journals Development and evaluation of tailored specific real-time RT-PCR assays for detection of foot-and-mouth disease virus serotypes circulating in East Africa

2016 ◽  
Vol 237 ◽  
pp. 114-120 ◽  
Author(s):  
Katarzyna Bachanek-Bankowska ◽  
Herieth R. Mero ◽  
Jemma Wadsworth ◽  
Valerie Mioulet ◽  
Raphael Sallu ◽  
...  
Keyword(s):  
Real Time ◽  
East Africa ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Pcr Assays

Establishment and validation of two duplex one-step real-time RT-PCR assays for diagnosis of foot-and-mouth disease

2016 ◽  
Vol 235 ◽  
pp. 168-175 ◽  
Author(s):  
K. Gorna ◽  
A. Relmy ◽  
A. Romey ◽  
S. Zientara ◽  
S. Blaise-Boisseau ◽  
...  
Keyword(s):  
Real Time ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Foot And Mouth ◽  
One Step ◽  
Pcr Assays

Prevalence of Coxsackievirus A6 and Enterovirus 71 in Hand, Foot and Mouth Disease in Nanjing, China in 2013

2015 ◽  
Vol 34 (9) ◽  
pp. 951-957 ◽  
Author(s):  
Ya-Qian Hu ◽  
Guang-Cheng Xie ◽  
Dan-Di Li ◽  
Li-Li Pang ◽  
Jing Xie ◽  
...  
Keyword(s):  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Coxsackievirus A6 ◽  
Foot And Mouth

scholarly journals Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

2006 ◽  
Vol 37 (1) ◽  
pp. 121-132 ◽  
Author(s):  
Scott M. Reid ◽  
Satya Parida ◽  
Donald P. King ◽  
Geoffrey H. Hutchings ◽  
Andrew E. Shaw ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Echovirus 4 associated to hand, foot and mouth disease

2006 ◽  
Vol 48 (4) ◽  
pp. 197-199 ◽  
Author(s):  
Denise Hage Russo ◽  
Adriana Luchs ◽  
Bráulio Caetano Machado ◽  
Rita de Cássia Carmona ◽  
Maria do Carmo Sampaio Timenetsky
Keyword(s):  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Immunofluorescence Assay ◽  
Mouth Disease ◽  
Epidemiological Surveillance ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Enteroviral Infection ◽  
Two Samples ◽  
Foot And Mouth

Hand, foot and mouth disease (HFMD) is a contagious enteroviral infection occurring primarily in children and characterized by vesicular palmoplantar eruptions and erosive stomatitis. Echovirus 4 (EV-4) has been commonly associated with aseptic meningitis. The association of HFMD with EV-4 has not been reported previously. Two samples of a 14-month child who presented mild fever, sores in the mouth, rash with blisters on the palm of hands and soles of feet were sent to Enteric Viruses Laboratory of Adolfo Lutz Institute. Clinical samples were inoculated in three different cell lines, and those which presented cytopathic effect (CPE), were submitted to Indirect Immunofluorescence Assay (IFA) and "one step" RT-PCR. Agarose gel electrophoresis from RT-PCR product, showed a product with 437 bp, which is characteristic of Enterovirus group. Echovirus 4 was identified by IFA. Although HFMD is a viral infection associated mainly with Enterovirus 71 (HEV-71) and Coxsackievirus A16 (CV-A16), our results demonstrate a diversity of serotype related to HFMD and stress the importance of epidemiological surveillance to this disease and its complications.

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