Validation of in-vitro antifungal activity of thyme essential oil on Aspergillus niger and Penicillium paneum through application in par-baked wheat and sourdough bread

Background: Ammoides verticillata Briq is an aromatic and medicinal herb that has been widely used in folk medicine for treatment of several types of pathologies such as respiratory problems, colds, fever, headache, gastric disorders and renal infections. Objective: Essential oils have been subjected of numerous works for their antimicrobial and insecticidal properties which have been valued by hundreds of publications and patents. The main objective of this work was to evaluate the chemical composition, and the in vitro and in vivo antifungal, and insecticidal properties of essential oil of Ammoides verticillata. Methods: In this work, the air-dried aerial parts of Ammoides verticillata were hydrodistilled in a Clevenger-type apparatus. The essential oil isolated was analyzed using gas chromatography (GC) and mass spectrometry (GC/MS). The in vitro antifungal activity of the essential oil was investigated against four plant fungi using radial growth technique. The effect of the essential oil on disease development of olive caused by Penicillium crustosum and Aspergillus niger in the in vivo conditions. The fumigation test of the essential oil was evaluated against L3 stages, pupas and adults of Bactrocera oleae known as the olive fly. Results: The essential oil of A. verticillata was characterized mainly by carvacrol (44.3%), limonene (19.3%) and p-cymene (19.2%). The result of in vitro antifungal activity of essential oil showed an interesting antifungal inhibition against Alternaria alternata and Fusarium solani strains with percentage inhibition of 89%. Furthermore, oil of A. verticillata has demonstrated promising in vivo antifungal activity to control infections of olives caused by Aspergillus niger and Penicillium crustosum. In addition, the essential oil exhibited fumigation toxicity against Bactrocera oleae with mortality percentage of 100% at 2 μL/L air. Conclusion: The results showed that essential oil of A. verticillata has interesting biological activities, which suggests that oil have the potential to be used as biocide to protect olives of pathogenic fungi and pests.

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In Vitro Antifungal Activity of Polysulfides-Rich Essential Oil of Ferula Latisecta Fruits against Human Pathogenic Dermatophytes

Natural Product Communications ◽

10.1177/1934578x0800300929 ◽

2008 ◽

Vol 3 (9) ◽

pp. 1934578X0800300 ◽

Cited By ~ 2

Author(s):

Mehrdad Iranshahi ◽

Abdolmajid Fata ◽

Bahareh Emami ◽

Bibi Mohadeseh Jalalzadeh Shahri ◽

Bibi Sedigheh Fazly Bazzaz

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Dilution Method ◽

Agar Dilution Method ◽

Agar Dilution ◽

Sulfur Containing ◽

Type Strains ◽

In Vitro Antifungal Activity

The increase in dermatophytoses and the fact that some patients do not respond well to therapy make it necessary to find new antifungal agents. As part of our ongoing studies on medicinal plants from Iran, we studied antidermatophytic activities of Ferula latisecta essential oil, which had shown considerable antifungal activity in preliminary antimicrobial screening. Antifungal activity was evaluated by determination of MIC values using the agar dilution method on type strains of Candida albicans and dermatophytes. The composition of the oil was characterized by GC and GC/MS analyses. The essential oil was rich in polysulfides (75.2%) and exhibited good activity against Trichophyton rubrum and T. verrucosom for about three weeks, with a MIC value 96 μg/mL. The oil showed antifungal activity, especially against dermatophytes, and the activity is probably related to the sulfur-containing components of the oil. This study has identified that the polysulfides-rich essential oil of Ferula latisecta fruits has activity against a range of human pathogenic dermatophytes, justifying future clinical trials to validate its use as a therapeutic alternative for dermatophytosis.

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Essential oil of Xylopia aethiopica from Cameroon: Chemical composition, antiradical and in vitro antifungal activity against some mycotoxigenic fungi

Journal of King Saud University - Science ◽

10.1016/j.jksus.2017.09.011 ◽

2018 ◽

Vol 30 (4) ◽

pp. 466-471 ◽

Cited By ~ 4

Author(s):

Alphonse Sokamte Tegang ◽

Thierry Marcel Ntsamo Beumo ◽

Pierre Michel Jazet Dongmo ◽

Leopold Tatsadjieu Ngoune

Keyword(s):

Chemical Composition ◽

Antifungal Activity ◽

Essential Oil ◽

Mycotoxigenic Fungi ◽

Xylopia Aethiopica ◽

In Vitro Antifungal Activity

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In vitro antifungal activity of Otacanthus azureus (Linden) Ronse essential oil alone and in combination with azoles

Planta Medica ◽

10.1055/s-0032-1320943 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

E Houël ◽

A Rodrigues ◽

A Jahn-Oyac ◽

JM Bessière ◽

V Eparvier ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

In Vitro Antifungal Activity

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Antifungal Activity of the Essential Oils from Ocimum gratissimum L. Grown in Togo

Journal of Scientific Research ◽

10.3329/jsr.v1i1.1131 ◽

2008 ◽

Vol 1 (1) ◽

pp. 164-171 ◽

Cited By ~ 11

Author(s):

Koffi Koba ◽

P W Poutouli ◽

Christine Raynaud ◽

Komla Sanda

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Filamentous Fungi ◽

Essential Oil Composition ◽

Oil Composition ◽

Minimum Fungicidal Concentration ◽

Ocimum Gratissimum ◽

Aerial Parts ◽

In Vitro Antifungal Activity

The aerial parts of Ocimum gratissimum L. (Lamiaceae) harvested in Togo was steam-distilled and investigated for essential oil composition (GC and GC/MS) and in vitro antifungal activities. Thymol (31.79 %), p-cymene (15.57 %) and Î³-terpinene (12.34 %) and were the major components of the oil. Other notable components identified in this oil were myrcene (6.94 %) and Î±-thujene (6.11 %).The in vitro antifungal activity was recorded with the minimum inhibitory concentrations (MICs) ranging from 80 to 150Â Âµl.l-1, 150 to 500 Âµl.l-1 Â and from 100 to 150 Âµl.l-1 respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Likewise, on tested fungi the minimum fungicidal concentration (MFC) varied from 300 Âµl.l-1 to 500 Âµl.l-1, 500 to 700 Âµl.l-1 and from 250 to 300 Âµl.l-1, respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Keywords: O.gratissimum,Â Antifungal, Essential oil; Thymol.Â Â© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.Â DOI: 10.3329/jsr.v1i1.1131Â

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Chemical Composition and Antifungal Activity of the Essential Oil of Cotinus coggygria from Marche Region (Italy)

Natural Product Communications ◽

10.1177/1934578x1801300919 ◽

2018 ◽

Vol 13 (9) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Daniele Fraternale ◽

Donata Ricci

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Pathogenic Fungi ◽

Gas Chromatography Mass Spectrometry ◽

Dilution Method ◽

Agar Dilution Method ◽

Plant Pathogenic Fungi ◽

Cotinus Coggygria ◽

In Vitro Antifungal Activity

The present study reports the results of gas chromatography-mass spectrometry (GC/MS) analyses of the essential oil from flowering aerial parts of Cotinus coggygria Scoop. (Anacardiaceae), as well as its in vitro antifungal activity against nine plant pathogenic fungi. Moreover, the essential oil was evaluated for its antifungal activity using the agar dilution method, and also MICs (minimum inhibitory concentrations) and MFCs (minimum fungicidal concentrations) were determined. The major compounds identified by GC-MS were limonene (49.2%), (Z)-β-ocimene (13.6%), α-pinene (8.8%) and (E)-β-ocimene (5.9%). The oil showed in vitro antifungal activity against some species of the Fusarium genus, Botrytis cinerea, and Alternaria solani. Our study indicates that the oil of C. coggygria could be used as a control agent for plant pathogenic fungi in natural formulations.

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