scholarly journals Hydrogeomorphic influences on salt marsh sediment accumulation and accretion in two estuaries of the U.S. Mid-Atlantic coast

2017 ◽  
Vol 383 ◽  
pp. 132-145 ◽  
Author(s):  
B.M. Boyd ◽  
C.K. Sommerfield ◽  
T. Elsey-Quirk
Keyword(s):  
Salt Marsh ◽  
Atlantic Coast ◽  
Sediment Accumulation ◽  
Marsh Sediment ◽  
Salt Marsh Sediment ◽  
The U.S

scholarly journals Spatially-resolved correlative microscopy and microbial identification reveals dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment

2020 ◽  
Author(s):  
Jeffrey Marlow ◽  
Rachel Spietz ◽  
Keun-Young Kim ◽  
Mark Ellisman ◽  
Peter Girguis ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Microbial Communities ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Community Members ◽  
Marsh Sediment ◽  
Anabolic Activity ◽  
Salt Marsh Sediment ◽  
Rrna Gene Sequencing ◽  
And Function

AbstractCoastal salt marshes are key sites of biogeochemical cycling and ideal systems in which to investigate the community structure of complex microbial communities. Here, we clarify structural-functional relationships among microorganisms and their mineralogical environment, revealing previously undescribed metabolic activity patterns and precise spatial arrangements within salt marsh sediment. Following 3.7-day in situ incubations with a non-canonical amino acid that was incorporated into new biomass, samples were embedded and analyzed by correlative fluorescence and electron microscopy to map the microscale arrangements of anabolically active and inactive organisms alongside mineral grains. Parallel sediment samples were examined by fluorescence-activated cell sorting and 16S rRNA gene sequencing to link anabolic activity to taxonomic identity. Both approaches demonstrated a rapid decline in the proportion of anabolically active cells with depth into salt marsh sediment, from ∼60% in the top cm to 10-25% between 2-7 cm. From the top to the bottom, the most prominent active community members shifted from sulfur cycling phototrophic consortia, to sulfate-reducing bacteria likely oxidizing organic compounds, to fermentative lineages. Correlative microscopy revealed more abundant (and more anabolically active) organisms around non-quartz minerals including rutile, orthoclase, and plagioclase. Microbe-mineral relationships appear to be dynamic and context-dependent arbiters of biogeochemical cycling.Statement of SignificanceMicroscale spatial relationships dictate critical aspects of a microbiome’s inner workings and emergent properties, such as evolutionary pathways, niche development, and community structure and function. However, many commonly used methods in microbial ecology neglect this parameter – obscuring important microbe-microbe and microbe-mineral interactions – and instead employ bulk-scale methodologies that are incapable of resolving these intricate relationships.This benchmark study presents a compelling new approach for exploring the anabolic activity of a complex microbial community by mapping the precise spatial configuration of anabolically active organisms within mineralogically heterogeneous sediment through in situ incubation, resin embedding, and correlative fluorescence and electron microscopy. In parallel, active organisms were identified through fluorescence-activated cell sorting and 16S rRNA gene sequencing, enabling a powerful interpretive framework connecting location, identity, activity, and putative biogeochemical roles of microbial community members.We deploy this novel approach in salt marsh sediment, revealing quantitative insights into the fundamental principles that govern the structure and function of sediment-hosted microbial communities. In particular, at different sediment horizons, we observed striking changes in the proportion of anabolically active cells, the identities of the most prominent active community members, and the nature of microbe-mineral affiliations. Improved approaches for understanding microscale ecosystems in a new light, such as those presented here, reveal environmental parameters that promote or constrain metabolic activity and clarify the impact that microbial communities have on our world.

scholarly journals Simple Metrics Predict Salt‐Marsh Sediment Fluxes

2019 ◽  
Vol 46 (21) ◽  
pp. 12250-12257 ◽  
Author(s):  
Daniel J. Nowacki ◽  
Neil K. Ganju
Keyword(s):  
Salt Marsh ◽  
Marsh Sediment ◽  
Sediment Fluxes ◽  
Salt Marsh Sediment

scholarly journals Salt marsh sediment bacteria: their distribution and response to external nutrient inputs

2009 ◽  
Vol 3 (8) ◽  
pp. 924-934 ◽  
Author(s):  
Jennifer L Bowen ◽  
Byron C Crump ◽  
Linda A Deegan ◽  
John E Hobbie
Keyword(s):  
Salt Marsh ◽  
Nutrient Inputs ◽  
Marsh Sediment ◽  
Salt Marsh Sediment ◽  
Sediment Bacteria ◽  
External Nutrient

Phylogenetic and Physiological Diversity of Sulphate-Reducing Bacteria Isolated from a Salt Marsh Sediment

1998 ◽  
Vol 21 (4) ◽  
pp. 557-568 ◽  
Author(s):  
Juliette N. Rooney-Varga ◽  
Barbara R. Sharak Genthner ◽  
Richard Devereux ◽  
Stephanie G. Willis ◽  
Stephanie D. Friedman ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Marsh Sediment ◽  
Sulphate Reducing Bacteria ◽  
Physiological Diversity ◽  
Salt Marsh Sediment ◽  
Reducing Bacteria

Metal(oid) mobility in a hypersaline salt marsh sediment (Sečovlje Salina, northern Adriatic, Slovenia)

2018 ◽  
Vol 644 ◽  
pp. 350-359
Author(s):  
Nives Kovač ◽  
Neli Glavaš ◽  
Teja Ramšak ◽  
Matej Dolenec ◽  
Nastja Rogan Šmuc
Keyword(s):  
Salt Marsh ◽  
Northern Adriatic ◽  
Marsh Sediment ◽  
Salt Marsh Sediment
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