scholarly journals Mycobacterioses in dogs and cats from Buenos Aires, Argentina

2017 ◽  
Vol 29 (5) ◽  
pp. 729-732 ◽  
Author(s):  
Soledad Barandiaran ◽  
Marcela Martínez Vivot ◽  
Elvira Falzoni ◽  
María J. Marfil ◽  
Gabriela Pérez Tort ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Tandem Repeat ◽  
Potential Risk ◽  
Buenos Aires ◽  
Variable Number Tandem Repeat ◽  
Clinical Signs ◽  
Variable Number ◽  
Molecular Testing ◽  
Full Characterization

Mycobacterioses can produce nonspecific clinical signs in dogs and cats that make diagnosis difficult. Furthermore, the full characterization of mycobacterial agents is not always possible or practical. We characterized mycobacteria detected through cytology in 12 dogs and 7 cats with generalized clinical signs from the province of Buenos Aires in Argentina. In dogs, molecular testing confirmed the presence of Mycobacterium avium subsp. hominissuis (MAH) in 8 cases and M. fortuitum in 1 case. All dogs were Miniature Schnauzers, suggesting that this breed may be more susceptible to M. avium than other dog breeds. The cat isolates were 2 M. bovis, 1 M. fortuitum, and 1 MAH. Mycobacterial interspersed repetitive unit–variable-number tandem repeat patterns suggested possible links with cattle, swine, and humans studied previously in Argentina. The results show that pets may act as susceptible hosts with the potential risk of transmitting the infection to humans and other animals.

scholarly journals Multiple-locus variable-number tandem repeat analysis and clinical characterization of Leptospira interrogans canine isolates

2015 ◽  
Vol 64 (3) ◽  
pp. 288-294 ◽  
Author(s):  
Nobuo Koizumi ◽  
Maki Mizutani Muto ◽  
Hidemasa Izumiya ◽  
Motoi Suzuki ◽  
Makoto Ohnishi
Keyword(s):  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Leptospira Interrogans ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Multiple-Locus Variable-Number Tandem Repeat Analysis Reveals Genetic Relationships within Bacillus anthracis

2000 ◽  
Vol 182 (10) ◽  
pp. 2928-2936 ◽  
Author(s):  
P. Keim ◽  
L. B. Price ◽  
A. M. Klevytska ◽  
K. L. Smith ◽  
J. M. Schupp ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Tandem Repeat ◽  
Genetic Relationships ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Group Method ◽  
Multiple Alleles ◽  
Multiple Locus ◽  
Sequence Characterization

ABSTRACT Bacillus anthracis is one of the most genetically homogeneous pathogens described, making strain discrimination particularly difficult. In this paper, we present a novel molecular typing system based on rapidly evolving variable-number tandem repeat (VNTR) loci. Multiple-locus VNTR analysis (MLVA) uses the combined power of multiple alleles at several marker loci. In our system, fluorescently labeled PCR primers are used to produce PCR amplification products from eight VNTR regions in the B. anthracisgenome. These are detected and their sizes are determined using an ABI377 automated DNA sequencer. Five of these eight loci were discovered by sequence characterization of molecular markers (vrrC 1, vrrC 2,vrrB 1, vrrB 2, and CG3), two were discovered by searching complete plasmid nucleotide sequences (pXO1-aat and pXO2-at), and one was known previously (vrrA). MLVA characterization of 426 B. anthracis isolates identified 89 distinct genotypes. VNTR markers frequently identified multiple alleles (from two to nine), with Nei's diversity values between 0.3 and 0.8. Unweighted pair-group method arithmetic average cluster analysis identified six genetically distinct groups that appear to be derived from clones. Some of these clones show worldwide distribution, while others are restricted to particular geographic regions. Human commerce doubtlessly has contributed to the dispersal of particular clones in ancient and modern times.

scholarly journals Evidence of in utero infection by Mycobacterium avium subsp. paratuberculosis using Multiple-Locus Variable-number tandem-repeat Analysis:

2020 ◽  
Vol 57 (1) ◽  
pp. e161653
Author(s):  
Brenda Vasini Rosell ◽  
Bernardo Lagleyze ◽  
Claudia Morsella ◽  
Laura Mendez ◽  
Florencia Bresky ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Mycobacterium Avium Subsp ◽  
Multiple Locus ◽  
Repeat Analysis ◽  
Acid Fast Bacilli ◽  
Specific Medium ◽  
Mycobacterium Avium Subsp Paratuberculosis

A pregnant heifer with an advanced clinical stage of paratuberculosis was reported in a herd in Argentina. Thus, the animal was euthanized and samples of organs of the cow and its fetus was taken and cultured for bacteriology in specific medium. Tissues were analyzed by histopathology (hematoxylin-eosin and Ziehl-Neelsen staining). Histopathological analysis of the cow’s samples revealed the presence of lesions consistent with paratuberculosis, and Ziehl-Neelsen staining revealed the presence of acid-fast bacilli, whereas the fetal tissues showed absence of lesions but the presence of acid-fast bacilli by Ziehl-Neelsen staining. After growing in specific medium, colonies in tissues from both cow and fetus were positive for IS900-PCR, confirming the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Finally, the isolates were typed by Multiple-Locus Variable-number tandem-repeat Analysis (MLVA), which confirmedthe epidemiological link between them. This study is the first in Argentina to report the detection of MAP that shares an identical MLVA type in a pregnant cow and its fetus. The results of this study are consistent with previous reports and highlight the intra-uterine transmission of MAP as an important source of infection within herds.

scholarly journals Characterization of Dutch Staphylococcus aureus from bovine mastitis using a Multiple Locus Variable Number Tandem Repeat Analysis

2009 ◽  
Vol 136 (3-4) ◽  
pp. 277-284 ◽  
Author(s):  
Risma Ikawaty ◽  
E.C. Brouwer ◽  
M.D. Jansen ◽  
E. van Duijkeren ◽  
D. Mevius ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Bovine Mastitis ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis
Sign in / Sign up

Export Citation Format

Share Document