Regulation of the inducible nitric oxide synthase and sodium pump in type 1 diabetes

2007 ◽  
Vol 69 (2) ◽  
pp. 302-306 ◽  
Author(s):  
Zorica Zakula ◽  
Goran Koricanac ◽  
Biljana Putnikovic ◽  
Ljiljana Markovic ◽  
Esma R. Isenovic
Keyword(s):  
Nitric Oxide ◽  
Type 1 Diabetes ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Sodium Pump ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Ivana Resanovic ◽  
Zoran Gluvic ◽  
Bozidarka Zaric ◽  
Emina Sudar-Milovanovic ◽  
Aleksandra Jovanovic ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Type 1 Diabetes ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Inos Activity ◽  
Early Effects ◽  
Nitrite Nitrate ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

This study aimed at examining the early effects of hyperbaric oxygen therapy (HBOT) on inducible nitric oxide synthase (iNOS) activity/expression in lymphocytes of type 1 diabetes mellitus (T1DM) patients. A group of 19 patients (mean age: 63 ± 2.1) with T1DM and with the peripheral arterial disease were included in this study. Patients were exposed to 10 sessions of HBOT in the duration of 1 h to 100% oxygen inhalation at 2.4 ATA. Blood samples were collected for the plasma C-reactive protein (CRP), plasma free fatty acid (FFA), serum nitrite/nitrate, and serum arginase activity measurements. Expression of iNOS and phosphorylation of p65 subunit of nuclear factor-κB (NFκB-p65), extracellular-regulated kinases 1/2 (ERK1/2), and protein kinase B (Akt) were examined in lymphocyte lysates by Western blot. After exposure to HBOT, plasma CRP and FFA were significantly decreased (p<0.001). Protein expression of iNOS and serum nitrite/nitrate levels were decreased (p<0.01), while serum arginase activity was increased (p<0.05) versus before exposure to HBOT. Increased phosphorylation of NFκB-p65 at Ser536 (p<0.05) and decreased level of NFκB-p65 protein (p<0.001) in lymphocytes of T1DM patients were observed after HBOT. Decreased phosphorylation of ERK1/2 (p<0.05) and Akt (p<0.05) was detected after HBOT. Our results indicate that exposure to HBO decreased iNOS activity/expression via decreasing phosphorylation of ERK1/2 and Akt followed by decreased activity of NFκB.

Involvement of inducible nitric oxide synthase and estrogen receptor ESR2 (ERβ) in the vascular dysfunction in female type 1 diabetic rats

Life Sciences ◽  
2019 ◽  
Vol 216 ◽  
pp. 279-286 ◽  
Author(s):  
Simone Marcieli Sartoretto ◽  
Fernanda Fernandes Santos ◽  
Beatriz Pereira Costa ◽  
Graziela Scalianti Ceravolo ◽  
Rosângela Santos-Eichler ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Estrogen Receptor ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Vascular Dysfunction ◽  
Diabetic Rats ◽  
Female Type ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Linkage of the Human Inducible Nitric Oxide Synthase Gene to Type 1 Diabetes1

2001 ◽  
Vol 86 (6) ◽  
pp. 2792-2796
Author(s):  
Jesper Johannesen ◽  
Angeles Pie ◽  
Flemming Pociot ◽  
Ole Peter Kristiansen ◽  
Allan Ertmann Karlsen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Immunohistochemical Analysis of Cellular Infiltrate and Gamma Interferon, Interleukin-12, and Inducible Nitric Oxide Synthase Expression in Leprosy Type 1 (Reversal) Reactions before and during Prednisolone Treatment

2001 ◽  
Vol 69 (5) ◽  
pp. 3413-3417 ◽  
Author(s):  
David Little ◽  
Saroj Khanolkar-Young ◽  
Anne Coulthart ◽  
Sujai Suneetha ◽  
Diana N. J. Lockwood
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Immunohistochemical Analysis ◽  
Gamma Interferon ◽  
Interleukin 12 ◽  
Prednisolone Treatment ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

ABSTRACT The effects of prednisolone treatment on the cellularity and cytokine (gamma interferon, interleukin-12, and inducible nitric oxide synthase) profiles of leprosy skin type 1 (reversal) reactions were studied using immunohistochemistry. Skin biopsies were taken from 15 patients with leprosy type 1 (reversal) reactions at days 0, 7, 28, and 180 after the start of steroid treatment. Prednisolone treatment had little effect at day 7, but by day 28 significant decreases were found in cytokine levels. Some patients maintained cytokine production at days 28 and 180. These results illustrate the strong Th1 profile of type 1 reactional lesions, the slow response to steroid therapy, and continuing activity at 180 days.

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