Preparation and characterization of porous anion-exchange membrane adsorbers with high protein-binding capacity

2008 ◽  
Vol 315 (1-2) ◽  
pp. 155-163 ◽  
Author(s):  
Dongming He ◽  
Mathias Ulbricht
Keyword(s):  
Protein Binding ◽  
Anion Exchange ◽  
Binding Capacity ◽  
Anion Exchange Membrane ◽  
High Protein ◽  
High Protein Binding ◽  
Protein Binding Capacity ◽  
Membrane Adsorbers ◽  
Exchange Membrane

Preparation and characterization of magnetic polymer nanospheres with high protein binding capacity

2005 ◽  
Vol 293 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Xianqiao Liu ◽  
Yueping Guan ◽  
Huizhou Liu ◽  
Zhiya Ma ◽  
Yu Yang ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Capacity ◽  
High Protein ◽  
High Protein Binding ◽  
Protein Binding Capacity ◽  
Polymer Nanospheres

scholarly journals Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 181
Author(s):  
Solomon Mengistu Lemma ◽  
Cristiana Boi ◽  
Ruben G. Carbonell
Keyword(s):  
Ion Exchange ◽  
Anion Exchange ◽  
Binding Capacity ◽  
Dynamic Binding ◽  
Host Cell Proteins ◽  
High Protein Binding ◽  
Protein Binding Capacity ◽  
Flow Through ◽  
Exchange Membrane ◽  
Culture Supernatants

This study presents the preparation and characterization of UV-grafted polybutylene terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of biomolecules. The PBT nonwoven was functionalized with sulfonate and secondary amine for cation and anion exchange (CEX and AEX), respectively. The anion exchange membrane showed an equilibrium static binding capacity of 1300 mg BSA/g of membrane, while the cationic membranes achieved a maximum equilibrium binding capacity of over 700 mg hIgG/g of membrane. The CEX and AEX membranes resulted in dynamic binding capacities under flow conditions, with a residence time of 0.1 min, of 200 mg hIgG/mL of membrane and 55 mg BSA/mL of membrane, respectively. The selectivity of the PBT-CEX membranes was demonstrated by purifying antibodies and antibody fragments (hIgG and scFv) from CHO cell culture supernatants in a bind-an-elute mode. The purity of the eluted samples exceeded 97%, with good log removal values (LRV) for both host cell proteins (HCPs) and DNA. The PBT-AEX nonwoven membranes exhibited a DNA LRV of 2.6 from hIgG solutions in a flow-through mode with little loss of product. These results indicate that these membranes have significant potential for use in downstream purification of biologics.

Ceftriaxone Distribution and Protein Binding between Maternal Blood and Milk Postpartum

1993 ◽  
Vol 27 (3) ◽  
pp. 294-297 ◽  
Author(s):  
Philippe Bourget ◽  
Valérie Quinquis-Desmaris ◽  
Hervé Fernandez
Keyword(s):  
Protein Binding ◽  
Adverse Reactions ◽  
Binding Capacity ◽  
Maternal Blood ◽  
High Protein ◽  
Initial Growth ◽  
Data Synthesis ◽  
Pregnancy And Postpartum ◽  
High Protein Binding ◽  
Protein Binding Capacity

OBJECTIVE: To study postpartum distribution and protein binding of ceftriaxone (CTX) between maternal blood and milk and to discuss risk factors and possible impact for the neonate. DATA SOURCES: Reference articles and books are identified in the text. DATA SYNTHESIS: CTX distribution and protein binding between maternal blood and milk postpartum were studied in a patient at term presenting with acute pyelonephritis caused by Escherichia coli. The antibiotic therapy prescribed pending bacteriology results consisted of CTX 2 g/d, ornidazole 1 g/d, and tobramycin 3 mg/kg/d. Pharmacokinetics of total CTX were studied after the first 2-g infusion. At plateau (i.e., two days after delivery; seventh infusion), pharmacokinetics and milk distribution of total and free CTX also were studied. No accumulation of CTX was noted in the plasma at plateau. When high dosages of CTX are used (∼2g), its penetration into the milk is important (i.e., protein binding capacity is overwhelmed). No notable adverse reactions occurred in mother or child. Thus, an important diffusion into the milk (4.4 percent of the dose) appears not to be clinically important. Our knowledge of both metabolism and milk distribution of drugs with high protein binding (≥95 percent) and an acid characteristic should be expanded to better understand their use during both the pregnancy and postpartum periods. Finally, the child of the patient described here has normal initial growth and development at the present time. CONCLUSIONS: Caution should be taken when drugs such as CTX, which have both high protein binding (≥95 percent) and an acid characteristic are administered to breastfeeding women. Drugs of this type should be systematically investigated to better understand their use during pregnancy and postpartum.

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