scholarly journals Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 181
Author(s):  
Solomon Mengistu Lemma ◽  
Cristiana Boi ◽  
Ruben G. Carbonell
Keyword(s):  
Ion Exchange ◽  
Anion Exchange ◽  
Binding Capacity ◽  
Dynamic Binding ◽  
Host Cell Proteins ◽  
High Protein Binding ◽  
Protein Binding Capacity ◽  
Flow Through ◽  
Exchange Membrane ◽  
Culture Supernatants

This study presents the preparation and characterization of UV-grafted polybutylene terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of biomolecules. The PBT nonwoven was functionalized with sulfonate and secondary amine for cation and anion exchange (CEX and AEX), respectively. The anion exchange membrane showed an equilibrium static binding capacity of 1300 mg BSA/g of membrane, while the cationic membranes achieved a maximum equilibrium binding capacity of over 700 mg hIgG/g of membrane. The CEX and AEX membranes resulted in dynamic binding capacities under flow conditions, with a residence time of 0.1 min, of 200 mg hIgG/mL of membrane and 55 mg BSA/mL of membrane, respectively. The selectivity of the PBT-CEX membranes was demonstrated by purifying antibodies and antibody fragments (hIgG and scFv) from CHO cell culture supernatants in a bind-an-elute mode. The purity of the eluted samples exceeded 97%, with good log removal values (LRV) for both host cell proteins (HCPs) and DNA. The PBT-AEX nonwoven membranes exhibited a DNA LRV of 2.6 from hIgG solutions in a flow-through mode with little loss of product. These results indicate that these membranes have significant potential for use in downstream purification of biologics.

In-situ Ion-Exchange Preparation and Topological Transformation of Trimetal-Organic Frameworks for Efficient Electrocatalytic Water Oxidation

2021 ◽  
Author(s):  
Bao Yu Xia ◽  
Ya Yan ◽  
Xianying Wang ◽  
Yuan Kong ◽  
Jiangwei Zhang ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Ion Exchange ◽  
Anion Exchange ◽  
Water Oxidation ◽  
Water Electrolysis ◽  
Anion Exchange Membrane ◽  
Topological Transformation ◽  
Exchange Membrane ◽  
Kinetics Of

Anion exchange membrane water electrolysis (AEMWE) with non-precious catalysts offers a promising route for industrial hydrogen production. However, the sluggish kinetics of anodic water oxidation hinder its efficiency and cost....

scholarly journals Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality

2019 ◽  
Vol 20 (1) ◽  
pp. 161 ◽  
Author(s):  
Tuhidul Islam ◽  
Amith D. Naik ◽  
Yasuhiro Hashimoto ◽  
Stefano Menegatti ◽  
Ruben G. Carbonell
Keyword(s):  
Binding Capacity ◽  
Protein A ◽  
Chinese Hamster ◽  
High Capacity ◽  
Alkaline Solutions ◽  
Peptide Ligand ◽  
Dynamic Binding ◽  
Ligand Density ◽  
Host Cell Proteins ◽  
Igg Binding

This work presents the use of peptide ligand HWRGWV and its cognate sequences to develop affinity adsorbents that compete with Protein A in terms of binding capacity and quality of the eluted product. First, the peptide ligand was conjugated to crosslinked agarose resins (WorkBeads) at different densities and using different spacer arms. The optimization of ligand density and display resulted in values of static and dynamic binding capacity of 85 mg/mL and 65 mg/mL, respectively. A selected peptide-WorkBeads adsorbent was utilized for purifying Mabs from Chinese Hamster Ovary (CHO) cell culture supernatants. The peptide-WorkBeads adsorbent was found able to withstand sanitization with strong alkaline solutions (0.5 M NaOH). The purity of the eluted product was consistently higher than 95%, with logarithmic removal value (LRV) of 1.5 for host cell proteins (HCPs) and 4.0 for DNA. HCP clearance was significantly improved by adding a post-load washing step with either 0.1 M Tris HCl pH 9 or 1 M NaCl. The cognate peptide of HWRGWV, constructed by replacing arginine (R) with citrulline, further increased the HCP LRV to 2.15. The peptide-based adsorbent also showed a remarkable performance in terms of removal of Mab aggregates; unlike Protein A, in fact, HWRGWV was found to bind only monomeric IgG. Collectively, these results demonstrate the potential of peptide-based adsorbents as alternative to Protein A for the purification of therapeutic antibodies.

Determination of ion-exchange equilibrium coefficient for a MA-41 anion-exchange membrane in sodium carbonate/hydrocarbonate solutions

Desalination ◽  
2002 ◽  
Vol 149 (1-3) ◽  
pp. 459-464 ◽  
Author(s):  
Elena Volodina ◽  
Yurii Senik ◽  
Oksana Basova ◽  
Natalia Pismenskaya ◽  
Victor Nikonenko ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Anion Exchange ◽  
Sodium Carbonate ◽  
Anion Exchange Membrane ◽  
Exchange Equilibrium ◽  
Exchange Membrane

scholarly journals A multiscale-pore ion exchange membrane for better energy efficiency

2018 ◽  
Vol 6 (17) ◽  
pp. 7714-7723 ◽  
Author(s):  
Hyukjin J. Kwon ◽  
Bumjoo Kim ◽  
Geunbae Lim ◽  
Jongyoon Han
Keyword(s):  
Energy Efficiency ◽  
Fluid Flow ◽  
Ion Exchange ◽  
Depletion Region ◽  
Ion Exchange Membrane ◽  
Flow Through ◽  
Exchange Membrane ◽  
Ion Depletion

A novel multiscale-pore (MP) ion exchange membrane (IEM) is proposed to eliminate the ionic resistance caused by the ion-depletion region (Rd), by allowing a fluid flow through the MP-IEM.

scholarly journals Effect of ionic strength on the ion exchange equilibrium between AMX membrane and electrolyte solutions

2015 ◽  
Vol 51 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Islem Louati ◽  
Fatma Guesmi ◽  
Akram Chaabouni ◽  
Chiraz Hannachi ◽  
Béchir Hamrouni
Keyword(s):  
Ionic Strength ◽  
Ion Exchange ◽  
Anion Exchange ◽  
Natural Waters ◽  
Electrolyte Solutions ◽  
Anion Exchange Membrane ◽  
Exchange Equilibrium ◽  
Thermodynamic Constants ◽  
Separation Factors ◽  
Exchange Membrane

The effect of ionic strength variation on the ion exchange equilibrium between AMX anion exchange membrane and electrolyte solutions containing the most dominant anions on natural waters (Cl−, NO3−, and SO42−) was studied. All experiments were carried out at a constant temperature of 25 °C. Ion exchange isotherms were established, at different ionic strengths from 0.1 to 0.5 M, for the systems (Cl−/NO3−), (Cl−/SO42−) and (NO3−/SO42−). Obtained results showed that for I = 0.1 M the affinity order is SO42 −>NO3−>Cl−. For I = 0.2 M this order is NO3−>SO42−>Cl−. For 0.3 and 0.5 M the AMX membrane becomes more selective for chloride than for nitrate or sulfate. Selectivity coefficients KNO3−Cl−, K2Cl−SO42− and K2NO3−SO42−, thermodynamic constants, and separation factors were calculated and decreased with the increase of ionic strength.

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