Invert emulsion: Method of preparation and application as proper formulation of entomopathogenic fungi

The aim of this work is the synthesis of calcium carbonate (CaCO3) nano and microparticles and their application as biomaterials (vehicles) for the sustained release of doxycycline. CaCO3 micro particles were synthesized by water-in oil (W/O) emulsion method using emulsion liquid membranes with bis (2-ethylhexyl) phosphate (D2EHPA) as carrier, Span 80 as surfactant, and toluene and kerosene as organic solvents. The aqueous phases contained 1 M CaCl2, and 1 M Na2CO3, respectively. The Dynamic Light Scattering (DLS) data showed CaCO3 particles with sizes ranging from around 100 nm to 3500 nm. The CaCO3 particles with the average diameters around 600 nm attained an adsorbtion of doxycycline of maximum 97.9%, and a slow and steady release with a cumulative value of approximative 50% after ten days.

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Evaluation of the growth and sporulation of different entomopathogenic fungi in different liquid and solid media at varied concentrations

International Journal of Bioassays ◽

10.21746/ijbio.2017.08.003 ◽

2017 ◽

Vol 6 (8) ◽

pp. 5459

Author(s):

Chandra Teja K. ◽

Rahman S. J.

Keyword(s):

Entomopathogenic Fungi ◽

Large Scale ◽

Insect Pests ◽

Culture Media ◽

Virulent Strain ◽

Carbon Sources ◽

Nitrogen Sources ◽

Maximum Growth ◽

Nutritional Requirements ◽

Virulent Strains

Entomopathogenic fungi like Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii are used in biological control of agricultural insect pests. Their specific mode of action makes them an effective alternative to the chemical Insecticides. Virulent strains of Entomopathogenic fungi are effectively formulated and used as bio-insecticides world-wide. Amenable and economical multiplication of a virulent strain in a large scale is important for them to be useful in the field. Culture media plays a major role in the large-scale multiplication of virulent strains of Entomopathogens. Different substrates and media components are being used for this purpose. Yet, each strain differs in its nutritional requirements for the maximum growth and hence it is necessary to standardize the right components and their optimum concentrations in the culture media for a given strain of Entomopathogen. In the current study, three different nitrogen sources and two different carbon sources were tried to standardize the mass multiplication media for seven test isolates of Entomopathogenic fungi. A study was also conducted to determine the ideal grain media for the optimum conidial yields of the test isolates. Yeast extract was found to be the best Nitrogen source for the isolates. The isolates tested, differed in their nutritional requirements and showed variation in the best nitrogen and carbon sources necessary for their growth. Variation was also found in the optimum concentration of both the ingredients for the growth and sporulation of the isolates. In the solid-state fermentation study, rice was found to be the best grain for the growth of most of the fungi followed by barley. The significance of such a study in the development of an effective Myco-insecticide is vital and can be successfully employed in agriculture is discussed.

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Lethal effect of isolates of entomopathogenic fungi on adult insects of Trogoderma Versicolor (Coleoptera: Dermestidae)

Agricultural sciences ◽

10.22620/agrisci.2016.19.004 ◽

2016 ◽

Vol VIII (19) ◽

pp. 25-30

Author(s):

Slavimira Draganova ◽

Evdokia Staneva

Keyword(s):

Entomopathogenic Fungi ◽

Lethal Effect

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